兩種多重核酸檢測方法對多種呼吸道病原體的一致性分析

Consistency analysis of two multiplex PCR methods for detecting multiple respiratory pathogens

馮婉珊(FONG Un San) ； 吳健欣(NG Kin Ian) ； 李靜(LEI Cheng)

《鏡湖醫學》 21卷2期 (2021/12) Pp. 44-46+75

摘要

目的：分析液相芯片技術（NxTAG）和巢式多重聚合酶鏈反應（Polymerase Chain Reaction PCR）技術檢測呼吸道病原體的一致性。方法：收集鼻咽拭子樣本110例，使用液相芯片技術和巢式多重PCR技術檢測呼吸道病原體，採用卡方檢驗、McNemar及Kappa檢驗的統計方法，比較兩種方法檢出病原體的一致性。結果：在110例樣本中NxTAG的病原體檢出率為66.4%，巢式多重PCR的病原體檢出率為90.9%，兩種方法檢測結果的一致性為72.7%。結論：兩種測定方法均有其優勢和缺點，對於不同的病原體，兩者敏感性不同，巢式多重PCR的敏感性稍高，操作簡便快速，但成本較高，臨床醫生可根據實際情況選擇合適的試驗。

關鍵字

多重PCR ； 呼吸道病原體 ； 一致性

並列摘要

Objective: To compare the consistency of two multiplex methods: the NxTAG and the Nested multiplex PCR in the detection of respiratory viruses. Methods: The respiratory viruses were tested in 110 nasopharyngeal swab samples using NxTAG and the Nested multiplex PCR. The Chi-square, McNemar, and Kappa tests were employed to evaluate the consistency of the two methods. Results: The overall detection rate for viruses tested by two methods was 66.4% for NxTAG and 90.9% for Nested multiplex PCR. The consistency between the two multiplex assays was 72.7%. Conclusion: There are several advantages and disadvantages of both methods. The sensitivity for different pathogens of both methods is different. The sensitivity of nested multiplex PCR is slightly higher, the operation is fast and straightforward, but the cost is higher. Clinicians can choose the appropriate test according to the clinical condition.

並列關鍵字

Multiplex PCR ； Respiratory viruses ； Consistency