多元蛋白質組學分析農場雞糞厭氧醱酵產生沼氣之微生物生態系統

Meta-proteomics Analysis of Microbial Ecosystem in the Anaerobic Digestion of Chicken Manure in Biogas Production Farm

林志剛(Tze-Kang Lam)

指導教授 ： 吳世雄

共同指導教授 ： 羅禮強(Lee-Chiang Lo)

國立臺灣大學/理學院/化學研究所/博士(2021年)

https://doi.org/10.6342/NTU202101242

摘要

元蛋白質組學分析方法在厭氧消化生產沼氣中的應用具有很多優勢，但尚未完全發現。這項研究旨在從化學和生物學的角度，探討兩階段雞糞醱酵系統中沼氣產生的詳細過程。由厭氧消化的第一階段到第二階段代謝過程中，功能性蛋白質的歧異度及變化顯示參與兩階段厭氧消化的代謝反應不同。自鑑定出的功能蛋白可以解釋甲烷化反應所需的碳來源以及此厭氧消化系統累積氨的原因。本研究之元蛋白質組學數據顯示，由於氨累積造成的逆境和營養源的限制，雞糞醱酵系統主要以基質甲酸鹽和乙酸鹽經氫營養型甲烷化途徑生產甲烷。與傳統的元基因組分析不同，本研究不僅提供雞糞醱酵系統中參與的微生物物種名稱，更直接以鑑定的蛋白質證據揭示甲烷和二氧化碳的產生途徑，進一步研究雞糞的沼氣生產。本篇論文採用之分析方法，提供一個新的視角作為未來研究雞糞厭氧消化產生沼氣的參考。

關鍵字

元蛋白質組學分析 ； 雞糞 ； 代謝途徑 ； 兩階段醱酵系統 ； 氨抑制

並列摘要

Using a meta-proteomics approach for the analysis of biogas production from anaerobic digestion has many advantages that await to be fully uncovered. In this study, we aimed to investigate biogas production from a stable 2-stage chicken manure fermentation system via a chemical and biological perspective. The diversity and functional protein changes that occurred from the 1st to 2nd stage were a good indication of the differential metabolic processes in anaerobic digestion. The identified functional proteins highlighted and explained the causes of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, hydrogenotrophic methanogenic pathway was adopted as an indicator of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomics analysis, this study successfully identified not only the microorganisms’ names, but also the enzymes and pathways involved in the generation of methane and carbon dioxide in biogas production of chicken manure. This thesis uses an analysis approach on issues of anaerobic digestion of chicken manure materials in the production of biogas from another perspective which could provide an idea and framework for future researchers to adopt in the future.

並列關鍵字

Meta-proteomics analysis ； Chicken manure ； Metabolic pathway ； 2-Stage fermentation system ； Ammonia inhibition

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