離子與電刺激對於聚(3,4-乙烯二氧噻吩)的抗沾黏性質影響:寡乙二醇與磷酸膽鹼之比較

Ionic and Electrical Stimuli Effects on Antifouling Properties of Functionalized Poly(3,4-ethylenedioxythiophene): The Comparison between Oligo(Ethylene Glycol) and Phosphorylcholine

陳越(Yue Chen)

指導教授 ： 羅世強

國立臺灣大學/工學院/材料科學與工程學研究所/碩士(2018年)

https://doi.org/10.6342/NTU201800796

摘要

對於帶電表面而言，離子與外加電位對表面性質有重要的影響。抗沾黏材料,包括親水的乙二醇以及兩性離子高分子,可以有效抵抗蛋白質吸附以及細胞沾黏因此在生醫應用上扮演了很重要的角色，例如植入式醫療器材、生物感測器、藥物控制釋放以及組織工程。在此研究中，我們仔細地對比導電高分子嫁接乙二醇跟磷酸膽鹼官能基的抗沾黏性質在不同的離子與電位下的抗沾黏能力。在此研究中，我們努力去闡述兩者表面抗沾黏性質的不同。我們選用導電高分子3,4-乙烯二氧基噻吩(3,4-ethylenedioxythiophene, EDOT)結合磷酸膽鹼官能基和乙二醇官能基並用電化學聚合的方式，聚合成導電高分子薄膜。帶負電荷的牛血清蛋白與正電荷的溶菌酶在不同離子下的吸附與抗沾黏程度將由石英晶體微量天平-耗散偵測系統(quartz crystal microbalance with dissipation, QCM-D)監控，藉由石英震盪頻率的改變，來估量吸附在官能基表面之質量變化。此外我們也探討霍夫梅斯特序列(Hofmeister series)離子對於蛋白質在水中的狀態和高分子薄膜型態的影響。根據霍夫梅斯特序列，在我們的系統中導入不同種類和濃度的陰離子，透過改變水溶液中的陰離子種類和陰離子濃度，再藉由從QCM觀察到的蛋白質吸附量，來討論不同的陰離子對高分子表面性質和蛋白質吸附的關聯性。吸附在表面的陰離子可以有效地增加高分子表面的水層和降低蛋白質非特定吸附。最後我們進一步結合恆電位儀和石英晶體微量天平去做電化學石英晶體微量天平在不同的電位下去控制蛋白質的吸附與脫附行為。在這實驗中對於帶電表面系統的維持與再生提供初步的原理且對於生物電子產品的應用相當重要。

關鍵字

導電高分子 ； 抗沾黏性質 ； 電刺激 ； 蛋白質吸附 ； 兩性離子高分子 ； 石英晶體微量天平-耗散偵測系統(QCM-D) ； 霍夫梅斯特序列

並列摘要

For electrified surfaces, ions and applied potentials play major roles in controlling the surface properties. Antifouling materials such as poly(ethylene glycol) and zwitterionic polymers that resist nonspecific protein binding and cell adhesion play a key role in various biomedical applications. In this study, we investigated and compared the antifouling properties of conducting polymers grafted with oligo(ethylene glycol) groups and phosphorylcholine (PC) groups in the presence of different anions and applied potentials. Considerable effort has been made to illustrate the different effects of manipulating the antifouling properties of these two surfaces. We prepared polymer films by applying electropolymerization to two functionalized (3,4-ethylenedioxythiophene) polymers containing triethylene glycol and PC groups, respectively. A quartz crystal microbalance with dissipation (QCM-D) was employed to characterize the negatively charged bovine serum albumin and positively charged lysozyme adsorption as a function of ionic concentration in the presence of various Hofmeister anions. The frequency changes corresponded to the protein or ion adsorption/desorption behavior on the surface. The anions adsorbed on polymer films to effectively enhance the hydration layer of the polymer surface and reduce nonspecific protein binding. We further integrated a potentiostat with the QCM-D to control the protein adsorption/desorption behaviors by applying potentials, and we conducted an electrochemical QCM-D study. Most importantly, with the synergistic effect of ions and surface potential, a nearly fresh polymer surface was regenerated. Through the synergistic effect of ions and surface potential, a nearly fresh polymer surface was regenerated. This study describes principles to maintain and regenerate the antifouling properties of electrified surfaces, which are critical for implanted bioelectronics applications.

並列關鍵字

conducting polymer ； antifouling ； electrical stimuli ； protein binding ； zwitterionic polymer ； quartz crystal microbalance with dissipation (QCM-D) ； Hofmeister series

參考文獻

Reference

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