不同固醇類荷爾蒙對白蝦非特異性免疫系統和生理的影響

The Effects of Dietary Different Steroid Hormone on Nonspecific Immune System and Haemolymph Parameter of White Shrimp（Litopenaeus vannamei）

楊志秋(Chih-Chiu Yang)

指導教授 ： 陳秀男

共同指導教授 ： 冉繁華(Fan-Hua Nan)

國立臺灣大學/生命科學院/動物學研究研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00559

摘要

本實驗是利用17β-estradiol、Progesterone和Testosterone三種固醇類荷爾蒙，分別在飼料中添加濃度為2、8與32ppm，餵飼白蝦八天，探討固醇類荷爾蒙對白蝦GOT酵素活性（Glutamate oxidacetate transaminase activity）、GPT酵素活性（Glutamate pyruvate transaminase activity）、膽固醇（Cholesterol）、三酸甘油脂（Triglycerides）、PO酵素活性（Phenoloxidase activity, PO activity）、SOD酵素活性（Superoxide dismutase activity, SOD activity）與血球O2-產生能力（Superoxide anion productvity）的影響。
實驗結果發現，在實驗期間飼料中加添荷爾蒙的組別，白蝦血淋巴中GOT酵素活性及GPT酵素活性在12小時就高於控制組，且一直延續到實驗結束。膽固醇（Cholesterol）與三酸甘油脂（Triglycerides）在實驗結束時均高於控制組。PO酵素活性在實驗前期有高於控制組的現象，但到了實驗後期則與控制組之間無顯著差異。SOD酵素活性亦大於控制組，而O2-沒有顯著的影響。整體而言，白蝦對於固醇類荷爾蒙在實驗進行12小時後就產生反應，顯示固醇類荷爾蒙對於白蝦在短時間之內就會有生理上的效果。就非特異性免疫系統方面，固醇類荷爾蒙對呼吸爆之O2-沒有影響，但對proPO系統卻有顯著之激活能力。

關鍵字

免疫 ； 南美白蝦 ； 固醇類荷爾蒙

並列摘要

In this experiment 17β-estradiol, progesterone and testosterone at concentration of 2, 8 and 32 ppm in feed were investigated on the effects of variation of activities of glutamate oxidacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), cholesterol concentration, Triglycerides concentration, phenoloxidase (PO), superoxide dismutase (SOD) and the concentrations of the superoxide anion in the haemocyte of white shrimp (Litopenaeus vannamei).
The result shower that GOT and GPT activities in L. vannamei of experimental shrimp fed with different steroid hormone were higher than those of the control shrimps. Its mean hepatopancreas got damage. The result also showed that the concentration of cholesterol and Triglycerides in the experiment shrimps were higher than control during the course of the experiment. Although phenoloxidase activity is higher than control at early period of experiment. Superoxide dismutase activity was significant higher than control, but the superoxide anion production of haemocyte was no significant difference. It was concluded that L. vannamei showed a quickly reaction in physiology at 12 hours.

並列關鍵字

immune ； Litopenaeus vannamei ； steroid hormone

參考文獻

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