Nano-material technology was developed rapidly for the past two decades. Because of the high surface area of the nano-scale materials, it has been used for the fuel cells and lithium batteries researching in electrochemistry. We used different parameters for the Co3O4 nanowires growing, and directly grew on the top of screen-printed carbon electrodes as material of modified electrodes for increasing surface area to improve the electrochemical activity. It might affect Co3O4 nanowires growth such as magnetic fields, electrode surface smoothness,pre-oxidation and the concentration of NH4NO3. We discussed how the parameters influence Co3O4 nanowires on growth in this paper. We attempted to use the modified electrodes in glucose concentration analysis at last. We expect the non-enzymatic electrode use on blood glucose test have great progress.