- 學位論文
利用超臨界反溶劑法進行異抗壞血酸、沒食子酸丙酯及薑黃素微粒化
Micronization of D-isoascorbic Acid, Propyl Gallate and Curcumin by Supercritical Anti-solvent Method
摘要
摘要 在本研究中，利用超臨界反溶劑法(SAS)，成功地將抗氧化劑異抗壞血酸、沒食子酸丙酯及食用色素薑黃素進行微粒化。藉由物質的微粒化，期望能提高抗氧化劑的抗氧化能力。本研究中也會探討操作的溫度、壓力、溶液流速、溶液濃度及噴嘴管徑等參數，對於微粒化結果所造成的影響。 在第一部份的研究中，以異抗壞血酸為標的藥物，進行超臨界反溶劑法微粒化的參數效應探討。在溶劑為丙酮、操作溫度為35 ℃、壓力為100 bar、溶液流速為1 mL/min、溶液濃度為5 mg/mL、毛細管噴嘴內徑為200 μm時，有最佳的微粒化效果，可將異抗壞血酸的顆粒尺寸由原始藥物的101 μm，微小化到3.30 μm的大小。在抗氧化能力方面，微粒化之後的產物其抗自由基效率，約為原始藥物的1.64倍，有明顯的提升。 第二部分的研究，則是以沒食子酸丙酯為目標藥物，進行超臨界反溶劑法微粒化的參數效應探討。在丙酮為溶劑、操作在溫度為35 ℃、壓力為140 bar、溶液流速為1 mL/min、溶液濃度為30 mg/mL、毛細管噴嘴內徑為100 μm時有最佳的微粒化效果。能將沒食子酸丙酯的顆粒尺寸由原始藥物的531 μm，微小化到7.70 μm的大小。在抗氧化能力方面，微粒化之後的產物其抗自由基效率約為原始藥物的1.93倍，也有明顯的增加。 第三部分的研究，是針對食用色素薑黃素，進行超臨界反溶劑法的微粒化及參數效應探討。在乙酸乙酯為溶劑、操作在溫度為35 ℃、壓力為100 bar、溶液流速為5 mL/min、溶液濃度為12 mg/mL、毛細管噴嘴內徑為200 μm時有最佳的微粒化效果。薑黃素的顆粒尺寸由原始藥物的17.74 μm，微小化到2.78 μm的大小。而當以乙酸乙酯為溶劑、操作在溫度為35 ℃、壓力為100 bar、溶液流速為5 mL/min、溶液濃度為10 mg/mL、毛細管噴嘴內徑為200 μm時，可得到外觀形貌多數為圓球狀的顆粒。
並列摘要
In this study, micronization of antioxidant, including D-isoascorbic acid, propyl gallate, and natural colorant curcumin has been successfully performed by using the Supercritical Antisolvent (SAS) method. The aim of this research was to increase the antioxidant activity of antioxidant after the SAS process. The parameters such as temperature, pressure, solution flow rate, solution concentration and nozzle diameter were discussed in order to find the optimal operating condition for micronization. For D-isoascorbic acid, the optimal result was obtained under the following conditions: solvent = acetone, T = 35 ℃, P = 100 bar, solution flow rate = 1mL/min, concentration = 5 mg/mL, nozzle diameter = 200 μm. The mean particle size was decreased from 101 μm to 3.30 μm. In the antioxidant activity test, the antiradical efficiency was increased about 1.64 times. In the second part, for propyl gallate, the optimal result was obtained under the following conditions: solvent = acetone, T = 35 ℃, P = 140 bar, solution flow rate = 1mL/min, concentration = 30 mg/mL, nozzle diameter = 100 μm. The mean particle size was decreased from 531.13 μm to 7.70 μm. In the antioxidant activity test, the antiradical efficiency was increased about 1.93 times. For curcumin, the optimal result was obtained under the following conditions: solvent = ethyl acetate, T = 35 ℃, P = 100 bar, solution flow rate = 5 mL/min, concentration = 12 mg/mL, nozzle diameter = 200 μm. The mean particle size was decreased from 17.74 μm to 2.78 μm. When changing the concentration to 10 mg/mL and fixing other parameters, the spherical particles could be obtained.