螢光異硫氰酸鹽葡聚醣從聚乙烯醇水膠釋放之動力學研究

Study on the Release Kinetics of FITC-Dextran from PVA-Based Hydrogel

王廷璟(Ting-Ching Wang)

指導教授 ： 蔡偉博

國立臺灣大學/工學院/化學工程學研究所/碩士(2020年)

https://doi.org/10.6342/NTU202000473

摘要

由於聚合物-藥物共軛物可用於改變遞送藥物的性質，因此可將其與水凝膠結合以實現控制釋放。儘管先前的文獻研究了不同參數對釋放的影響，但其中多數是定性描述並且主要針對小分子藥物。
在這項研究中，調查了聚乙烯醇-酪胺(poly(vinyl alcohol)-tyramine, PVA-tyr)水凝膠中螢光異硫氰酸鹽葡聚醣(Fluorescein isothiocyanate–dextran, FITC-dextran)在不同條件下的釋放動力學。藉由使用數學模型來闡明水凝膠系統中聚合物-藥物共軛物的行為以及爆量釋放與水凝膠條件之間的關係。
結果顯示，填載量對釋放曲線除了爆量釋放的上升外幾乎沒有影響。水凝膠的厚度對較小的螢光異硫氰酸鹽葡聚醣具有較好的控制效果。相反，聚乙烯醇-酪胺高分子的取代度對較大分子量的共軛物具有更明顯的影響。改變分子大小顯示出顯著的分離效果，表明透過使用分子量控制釋放將是有效的策略。來自不同觀點的兩種數學分析也顯示，水凝膠中的釋放具有不同的時期和不同的部分，可能是由於水凝膠的異質結構所致。聚合物-藥物共軛物的混合釋放展現了使用混合尺寸的共軛物控制水凝膠的藥物釋放之可行性。這項研究可以用以促進設計聚合物-藥物共軛物在水凝膠系統中的控制釋放。

關鍵字

藥物輸送 ； 水凝膠 ； 聚合物-藥物共軛物 ； 建模 ； 控制釋放

並列摘要

Since the polymer-drug conjugate can be used to change the properties of the drug being delivered, it could be combined with hydrogels to achieve controlled release. Although previous research has studied the effect of various parameters on release, most of them were qualitative descriptions and focused on small molecule drugs.
In this study, the release kinetics of fluorescein isothiocyanate–dextran (FITC-dextran) from poly(vinyl alcohol) (PVA)-based hydrogels regarding different conditions were investigated. By applying mathematical models, the behavior of polymer-drug conjugates inside the hydrogel system was tried to be clarified. Meanwhile, the relationship between burst release and the hydrogel conditions was also concerned.
The results showed that the loading amount had little effect on the released profile, except for the rising burst ratio. The thickness of the hydrogel had better controlling effect on the smaller FITC-dextran; in contrast, the degrees of substitution of PVA-tyr had a more obvious effect on larger molecular weight conjugates. Changing the molecular size of the FITC-dextran showed a significant sieving effect, showing that controlling the release by using molecular weight would be an effective strategy. Two kinds of mathematical analysis from different views also showed that the release in hydrogel would have different periods and different parts, might due to the heterogeneous structure of hydrogel. The mixing release of polymer-drug conjugates demonstrated the feasibility of using mixed-size polymer-drug conjugates to control drug release with hydrogel. It is expected that this research can improve the design of controlled release with polymer-drug conjugates in hydrogel systems.

並列關鍵字

Dug delivery ； Hydrogel ； Polymer-drug conjugates ； Modeling ； Controlled release

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