以原位雜合反應探討雙磷酸鹽對骨骼再生之影響

Evaluating Effects of Bisphosphonates on Bone Regeneration in Zebrafish by whole-mount in situ hybridization.------- A Preliminary Study

廖于茹(Yu-Ju Liao)

指導教授 ： 王若松

共同指導教授 ： 張百恩(Bei-En Chang)

國立臺灣大學/牙醫專業學院/臨床牙醫學研究所/碩士(2012年)

https://doi.org/10.6342/NTU.2012.01429

摘要

無資料

關鍵字

雙磷酸鹽 ； 雙磷酸鹽相關顎骨壞死 ； 斑馬魚尾鰭再生 ； 基因表現 ； 間葉組織細胞增生及分化

並列摘要

Bisphosphonates are effective drugs widely used to treat osteoporosis, Paget's disease, osteogenesis imperfecta and skeletal complications caused by metastatic cancer. Although generally well tolerated, there is a rare but potentially severe side effect, bisphosphonate related osteonecrosis of the jaw (BRONJ), first described in 2003. The clinical manifestation of the disease is exposure of bone, which is frequently accompanied by pain, soft tissue swelling or ulceration, suppuration, and abscess formation. BRONJ significantly impairs the quality of life and complicates dental treatment, such as tooth extraction and dental implant restorations.
To date, the definitive mechanism of BRONJ still remains elusive. In this study, we use zebrafish as an in vivo animal model to dissect the etiology of BRONJ to unravel the underlying molecular mechanism using whole-mount in situ hybridization (ISH) on regenerative caudal fins after the amputation surgery.
Our preliminary results suggest that bisphosphonates (alendronate) have no significant effects on gene expression related to mesenchymal cell proliferation and differentiation during the early stage of fin regeneration. However, further studies are needed to characterize the possible effects of bisphophobates during the entire process of fin regeneration. Eventually, we expect to unravel the detailed mechanism of BRONJ and shed light on potential treatment of this disease.

並列關鍵字

Bisphosphonates ； BRONJ ； zebrafish fin regeneration ； whole-mount in situ hybridization (ISH) ； gene expression ； mesenchymal cells

參考文獻

吳俊學. (2010). 利用斑馬魚活體實驗來探討雙磷酸鹽造成顎骨壞死之可能機制.

Allen, M. R. and Burr, D. B. (2011). Bisphosphonate effects on bone turnover, microdamage, and mechanical properties: what we think we know and what we know that we don't know. Bone 49, 56-65.

Duque, G. and Rivas, D. (2007). Alendronate has an anabolic effect on bone through the differentiation of mesenchymal stem cells. J Bone Miner Res 22, 1603-11.

Eastell, R., Walsh, J. S., Watts, N. B. and Siris, E. (2011). Bisphosphonates for postmenopausal osteoporosis. Bone 49, 82-8.

Bisphosphonates influence the proliferation and the maturation of normal human osteoblasts