香草酸對餵食高脂飼料大鼠血糖與血脂之影響

Effect of Vanillic Acid on Blood Glucose and Blood Lipid in Rats Fed with High-fat Diet

陳甄雯(Chen-Wen Chen)

指導教授 ： 吳瑞碧

共同指導教授 ： 沈賜川

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.01379

摘要

台灣糖尿病人口逐年攀升，已躍居國人十大死因的第五位，其中糖尿病患者約有95 % 屬於第二型糖尿病，主因為胰島素阻抗而導致高血糖之症狀。酚酸廣泛存在於許多蔬果中，已有許多研究證實酚酸具有降血糖之生理活性，但目前仍未被完整的研究。本研究首先利用9種酚酸對小鼠肝臟上皮細胞株FL83B進行細胞存活率試驗，結果顯示，酚酸之最高安全使用劑量為12.5 μM，再以葡萄糖攝入試驗篩選具降血糖潛力之酚酸，香草酸在改善胰島素阻抗細胞葡萄糖攝入能力具有良好效果，接著以動物實驗探討香草酸對高脂飼料餵食大鼠之影響。實驗設計將雄性Sprague-Dawley大鼠隨機分為兩組，一組給予正常飲食 (脂肪佔總飲食熱量14 %)，另一組給予高脂飲食 (脂肪佔總飲食熱量60 %)，實驗期間定期監測大鼠禁食血糖變化；將餵食12週高脂飲食後成功誘導成高血糖動物模式之大鼠，再隨機分為負控制組、正控制組 (餵予pioglitazone) 及實驗組 (餵予香草酸)。結果顯示，以高脂飼料餵食大鼠12週後可成功誘發高血糖症狀，而每日餵食香草酸 (30 mg/kg b.w.) 4週後則能顯著改善高脂飲食大鼠高血糖、高血脂、高瘦體素血症及胰島素阻抗之現象。由上述結果顯示，香草酸可能具有改善胰島素阻抗及血脂異常之效果，有助於調節糖尿病前期之血糖異常，未來具有開發成健康食品及膳食補充劑之潛力。

關鍵字

高血糖 ； 胰島素阻抗 ； 酚酸 ； 香草酸 ； 高脂飲食

並列摘要

Diabetes mellitus (DM) is the 5th leading cause of death in Taiwan, and the prevalence is still growing. More than 95 % among all the cases belong to type 2 diabetes and characterized as insulin resistance that resulted in hyperglycemia. Phenolic acids are widely distributed in vegetables and fruits. Although some studies have shown the effect of phenolic acids on anti-hyperglycemia, there has been no systematic study on DM yet. In this study, the insulin resistant mouse hepatocytes FL83B cell model was established and used as the screen platform. The cell viability test showed that the critical safe dosage of 9 phenolic acids is 12.5 μM. The result from glucose uptake test showed that vanillic acid exhibits the highest increment in glucose uptake in insulin resistant cells among tested samples. Animal model was then performed to assess the effect of vanillic acid in high fat diet-fed rats. Male Sprague-Dawley rats are randomly divided into two groups, including a control group (fed with chow diet containing 14 % kcal from fat), and a high-fat diet group (fed with high-fat diet containing 60 % kcal from fat) for the development of hyperglycemia. Furthermore, the high-fat diet group were further divided into three sub-groups, including the high-fat diet sub-group, positive control sub-group (orally administrated pioglitazone, 30 mg/kg b.w.) and treatment sub-group (orally administrated vanillic acid, 30 mg/kg b.w.). The results showed that vanillic acid significantly alleviated high-fat diet induced syndrome, including hyperglycemia, hyperlipidemia, hyperleptinemia and insulin resistance. We thus demonstrated the potential of vanillic acid in the development of health foods or dietary supplements for the prevention of hyperglycemia in prediabetes.

並列關鍵字

Hyperglycemia ； insulin resistance ； phenolic acids ； vanillic acid ； high-fat diet

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