量測小鼠心房組織電生理之頻閃式光學影像映射系統研發

Development on a Stroboscopic Optical Mapping System for Murine Atrial Electrophysiological Measurement

張毓成(Yu-Cheng Chang)

指導教授 ： 陳亮嘉

國立臺灣大學/工學院/機械工程學研究所/碩士(2016年)

https://doi.org/10.6342/NTU201602701

摘要

光學影像映射系統是研究心臟電生理之重要工具，其非接觸動物心臟的光學量測為一大特點。心臟電生理之訊號變動相當快速且複雜，對於影像擷取技術及設備目前仍有許多限制，因此，在光強相對低之螢光且需高取像速度的限制條件下，傳統光學影像映射系統之取像裝置，空間解析度普遍相當低，且分析程式無法靈活調整，因此對於小動物心臟電生理研究，仍有許多限制及不足的地方。
心房各項電生理的變化為重要的指數，尤其對心房常見的顫動的引發，有著重要的生理意義，因此心房電生理一直為許多研究之目標，但常受制於量測系統而無法精確分析。由於小鼠心房組織面積小，相較心室對空間解析度之要求高，且螢光訊號微弱，使量測難度更甚。本研究成功開發出一個即時量測心房之光學影像映射系統，以觀測小鼠心房異常電生理訊號，並找出適合頻閃式量測之條件。同時，本研究成功實現將刺激訊號與取像訊號同步，使頻閃式取像更為準確，且利用頻閃式量測，有效降低取像速度之限制。此外，開發靈活調整之分析程式，使後續發展整合系統更為容易，本研究開發之雙模式(即時與頻閃)光學影像映射系統，可用於觀測及研究小鼠心房電生理特性。

關鍵字

光學影像映像 ； 小鼠心房電生理 ； 頻閃技術 ； 動作電位傳遞圖

並列摘要

Optical mapping system is one of the most important research tools in cardiac electrophysiology. Optical mapping is a non-contact investigation. Because cardiac electrophysiology signal changes very fast and in a complicated manner, the acquisition performance of the detecting camera becomes extremely high. In this condition, the spatial resolution of traditional optical mapping system normally become very low due to utilization of large optical sensors, and the measurement of electrophysiology in small size animal still have a lot of restrictions when the insufficient dynamic light response stops effective imaging detection.
Atrial fibrillation is a common and dangerous disease in clinic. Many scientists are focusing on atrial electrophysiology, but effective analyses are still restricted by insufficient detection resolution of the measuring system. The size of murine atria is small, so the requirement of spatial resolution is higher than the ventricle. The low intensity of fluorescence in the atrial of mice made the measurement rather difficult to do. The aim of the thesis is to develop a dual-mode optical mapping system for measuring murine atrial electrophysiology by both of the real-time mode for high-speed detection and the stroboscopic mode for achieving high optical magnification and high speed simultaneously. The research developed a real-time optical mapping system to determine the best condition of atrial electrophysiological images by using stroboscopic mode. This study realized the synchronization of stimulus signal and camera external trigger signal, so the developed system can improve the precision of optical mapping measurement in murine atrium by using stroboscopic mode. The stroboscopic mode optical mapping system can rely on low frame rate cameras for operation. A flexible signal analysis program was also developed to make the operation and analysis of the developed system easier. This research have developed a dual mode optical mapping system to observe and research of murine atrial electrophysiology.

並列關鍵字

Optical mapping ； Murine atrial electrophysiology ； Stroboscopic technique ； Activation map

參考文獻

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