培養液外泌體微米珠酵素結合免疫吸附三明治螢光分析方法之研究

Beads-based Sandwich ELISA for Fluorescent Detection of Exosomes from Cultured Medium

張哲睿(Che-Jui Chang)

指導教授 ： 胡文聰

國立臺灣大學/工學院/應用力學研究所/碩士(2022年)

https://doi.org/10.6342/NTU202202407

摘要

生物標記物在疾病管理中的應用在臨床上涵蓋了非常廣泛的範圍，包括疾病篩檢、診斷、臨床診斷指導建議、狀況監控等方面。隨著人們對於疾病在臨床階段前的篩檢或是特定濃度極低的生物標記物檢測的興趣提高，傳統的免疫分析方法需要有實質且重大的突破以達到需求。
本論文的目的是透過雙微米珠的三明治酵素結合免疫分析法實現ELISA 的結果。結果顯示在實驗設計方面提供了總體方法的初步驗證。這項工作為實驗中的每個單個步驟提供了一種定量分析的方法，並通過三明治ELISA給出了這種特殊方法的初步結果。需要未來的工作來進一步驗證其他參數。

關鍵字

外泌體 ； 蛋白檢測 ； 三明治酵素結合免疫分析

並列摘要

The use of biomarkers for disease management spans a wide clinical spectrum, including screening, diagnosis, therapeutic guidance and monitoring. As interests grow in detecting disease in the preclinical stage or with specific biomarkers with extremely low concentration, substantial breakthrough from the traditional immunoassay approach is needed.
The purpose of this thesis was to implement beads-based sandwich ELISA using micron-scaled particles observed by bright field and a fluorescence channel. Results provided preliminary validation in the overall approach in terms of experimental design. This work provides a method of quantitative analysis for each individual steps in experiments and gives preliminary results of this particular approach via sandwich ELISA. Future work is needed to further validate additional parameters.

並列關鍵字

Exosomes ； protein detection ； sandwich ELISA

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