溶解性微生物產物與胞外聚合物質之特性與過濾行為

Characteristics, Coagulation and Filtration of Soluble Microbial Products and Extracellular Polymeric Substances

蔡博年(Bor Nein Tsai)

指導教授 ： 李篤中

國立臺灣大學/工學院/化學工程學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.00873

摘要

本文章主要為從廢水處理之活性污泥萃取出的微生物溶解性產物（Soluble Microbial Products, SMP），鬆散鍵結性胞外聚合物質（Loosely Bound Extracellular Polymeric Substances, LBEPS）與緊密性鍵結胞外聚合物質（Tightly Bound Extracellular Polymeric Substances, TBEPS）中的溶解態有機物質（Dissolved Organic Matter, DOM），用尺寸排斥層析法（Size Exclusion Chromatography, SEC）依照分子量分類，利用紫外-可見光吸收光譜（波長230、254、280nm）、溶解性總有機碳（Total Dissolved Organic Carbon, DOC）與三維螢光（Excitation-Emission Matrix, EEM）作不同區間的特性分析。發現在SMP中分子量大於20000Da的有機物佔總體DOM的33%，芳香族類的蛋白質佔了其中大部分。LBEPS與TBEPS中DOM的組成大於20000Da的大分子約有22%，其組成份以腐質酸類的物質與蛋白質為主。多元氯化鋁（Polyaluminum Chloridel，PACl）可有效移除具大分子量之DOM。從EEM與UV吸收光譜可發現大分子量之蛋白質與腐質酸類在經過PACl加藥程序後被移除，但PACl無法移除分子量小於1000Da的DOM之低芳香性之有機酸類。由上述之實驗發現SMP與LBEPS之溶解態有機物的物理化學性質相似但不完全一樣，由SEC分離後之樣品經分析後發現TBEPS之組成與SMP及LBEPS相當不同。

並利用薄膜程序分析經過加藥條理後之水樣比較DOC之移除率與UV吸收光譜。發現經過UF膜（PES，0.22μm）DOC移除率可以達到80％以上，其中對薄膜阻塞的貢獻度以LBEPS最大。

關鍵字

微生物溶解性物質 ； 混凝

並列摘要

The dissolved organic matters (DOM) in the soluble microbial products (SMP), loosely bound extracellular polymeric substances (LBEPS), and tightly bound extracellular polymeric substances (TBEPS) extracted from a wastewater sludge were fractionated using size exclusion chromatography, and were characterized by ultraviolet absorbance at 230, 254, and 280 nm, the dissolved organic carbon (DOC) contents, and the excitation-emission-matrix (EEM) spectra. The DOM of molecular weight (MW) > 20000 Da accounted for 33% for SMP, which incorporated proteins of high aromaticity, and 22% for both LBEPS and TBEPS, which were composed of organic acids-like substances and proteins respectively. Polyaluminum chloride (PACl) coagulation could effectively remove this fraction of DOM. On the other hand, the DOM of MW< 1000 Da were mostly non-aromatic organic acids (and probably also polysaccharides) of low aromaticity for SMP and both EPS, and were hardly removed by PACl coagulation. Based on the EEM and UV absorbance data, proteins of high MW and humic substances were easily removed by PACl coagulation. The present study indicates that, although the chemical characteristics of DOM in SMP and LBEPS were alike, but not identical. The DOM in TBEPS differed significantly from SMP or LBEPS based either on DOC distributions or on EEM fingerprints.

並列關鍵字

SMP ； coagulation

參考文獻

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