多重染色方案-胞外聚合物於生物聚集體之分佈

Multiple Staining Scheme - Distribution of Extracellular Polymeric Substances in Bioaggregates

陳明源(Ming-Yuan Chen)

指導教授 ： 李篤中

國立臺灣大學/工學院/化學工程學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.01766

摘要

本論文提出結合雷射共軛焦顯微鏡(Confocal Laser Scanning Microscope)掃描且最高染劑數目達六個之多重染色方案，用來觀察生物聚集體中之胞外高分子(EPS)之分佈。藉由調整螢光收光範圍避免染劑螢光互相干擾，觀測生物聚集體中蛋白質、脂質、a-多醣、b-多醣及菌群分佈情形。
實驗利用不同數目染劑探討生物聚集體內EPS之分佈。螢光結果顯示EPS於薄膜上的不規則沈積造成過濾速度的下降。染色結果發現活性污泥膠羽(floc)內部EPS分佈相當均勻，然而好氧顆粒(aerobic granule)內呈現多層分佈，厭氧顆粒(anaerobic granule)中則以蛋白質為主要成分。實驗也針對生物顆粒之發展及其飢餓(starvation)實驗探討其內部EPS及菌群分佈。

關鍵字

生物顆粒 ； 共軛焦

並列摘要

This work proposed a multi-staining method with up to six stains combined with Confocal Laser Scanning Microscope (CLSM) scanning for probing the distributions of extracellular polymeric substances in bioaggregates. By carefully selecting the wavelength ranges collected, the distributions of proteins, lipids, a-and b-polysaccharides, and cells in bioaggregates were built up.
Different numbers of stains were used to probe the EPS in selected bioaggregates. Experimental observation revealed a rather uniform distribution of wastewater sludge flocs. However, multi-layer structure was noted for the aerobic granules. Proteins made up the majority body of anaerobic granules. This work also discussed the structural changes in granule development and their response to starvation.

並列關鍵字

granule ； confocal

參考文獻

蔡德耕，「活性污泥膠羽之氧氣輸送阻力」，碩士論文，國立台灣大學化學工程研究所，台北(2004)

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