摘要
生物顆粒(granule)是經由細胞自身與細胞外間質(extracellular polymeric substances, EPS)聚集而成近似圓球的生物聚集體。EPS可作細胞之間的架橋，具有強化生顆粒結構的功能，而EPS組成多為蛋白質、多醣類及脂質。研究指出，正二價及正三價金屬陽離子能加速顆粒形成，例如Ca2+。 本論文採用3種生物顆粒(granule)作為測試樣品，分別為好氧酚顆粒、厭氧產氫顆粒及厭氧產甲烷顆粒。顆粒中加入螫合劑EDTA、α- amylase、β- amylase、proteinase K、lipase、cellulase，藉由螢光探針雜交顆粒中的多醣體、脂質、蛋白質，結合雷射共軛焦顯微鏡(Confocal Laser Scanning Microscope)掃描，探討金屬陽離子、蛋白質、脂質、多醣體及纖維素對顆粒粒結構穩定性影響。 結果顯示金屬陽離子、多醣體、脂質及蛋白質對酚好氧顆粒、厭氧產氫顆粒及厭氧產甲烷顆粒結構強度不造成影響，纖維素對酚好氧顆粒及厭氧產甲烷顆粒結構強度不造成影響，但是纖維素是厭氧產氫顆粒網狀結構的支幹物質。
並列摘要
Granules can be described as a collection of sel-immobilized cells and extracellular polymeric substances (EPS) into a somewhat spherical form. EPS can mediate both cohesion and adhesion of cells and play a crucial role in maintaining the structural integrity in a community of immobilized cell and the composition of EPS include proteins, polysaccharides and lipid .In recently research, the presence of divalent and trivalent cations ions, such as Ca2 + etc, helps cells to form microbial nuclei that promote further granulation The investigation into structural stability of three biochemical granules (one aerobic granules and two anaerobic granules) were examined. Selective enzymes of proteins, lipids, proteinase K, α- amylase, β- amylase, cellulase and EDTA were added to those three granules to influence structural stability. The roles of proteins,α- and β-polysaccharides, and lipids were studied via their selective hydrolysis using enzymes and structural changes of granule were probed using in situ fluorescent staining and confocal laser scanning microscopy. The results show elective enzymes of proteins, lipids, proteinase K, α amylase, β amylase and EDTA are not backbone of those three granules.Also, cellulose are not backbone of methane production by anaerobic granules and Degradation of Phenol by Aerobic Granules. However, cellulose form the backbone of a network-like outer layer with embedded proteins, lipids, a-polysaccharides, and cells to support the mechanicalstability of structural integrity of hydrogen production by anaerobic granules.
參考文獻
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