以氧化鋅奈米柱增進靈敏度及特異性之薄膜電晶體蛋白質檢測器

在這篇論文中，我們以氧化鋅奈米柱為基礎，建立了癌症相關的檢測方法。我們透過實驗證明氧化鋅奈米柱能夠和抗體結合，具有特異性的抗體和氧化鋅奈米柱的組合能夠構成具有特異性的生物標記。研究指出癌細胞大量的表現表皮生長因子接受器，因此我們選擇了表皮細胞生長因子接受器的抗體來進行實驗。我們將表皮生長因子接受器的抗體和氧化鋅奈米柱結合製成癌細胞偵測標記，這個癌細胞標記可以透過表皮生長因子接受器和癌細胞接合，當氧化鋅奈米柱受到紫外光的照射時，會被激發出波長大約377奈米的紫光，我們便可以藉由光致激發螢光頻譜和影像來辨認出癌細胞。螢光染色檢測法是目前最普遍的癌症偵測技術，然而螢光衰退、螢光顯色限制以及細胞自體螢光分辨等，使得即時檢測相當困難。由氧化鋅奈米柱製成的癌細胞爭測標記解決了上述的問題，進而使得癌細胞能夠被即時檢測。除此之外，氧化鋅奈米柱也應用於薄膜電晶體蛋白質檢測器來提升感測器的敏感度。這個新型蛋白質解測器具有延伸的金感測板用來增加感測器的感測區域並且將待測的生物化學溶液和感測器分隔開來。我們接著用氧化鋅奈米柱和表皮生長因子接受器的抗體將感測板功能化，以進行表皮生長因子接受器的特異性量測。氧化鋅奈米柱的生物相容性以及高面積對體積比例大幅增加蛋白質和感測器的結合能力，進而大幅提升了感測器的敏感度。透過這個新型結構，我們實現了快速簡便並且具有高免感度及高特異性的感測過程。我們的薄膜電晶體蛋白質感測器可以量測到在0.1 ng/ml的細胞蛋白質溶液中36.2 fM的表皮生長因子接受器。

關鍵字

癌症；表皮生長因子接受器；氧化鋅奈米柱；高敏感度生物感測器

並列摘要

In this thesis, tumor related assays are introduced based on the properties of ZnO nanorods. ZnO nanorods are able to form biomarkers of specificity together with antibodies. EGFR antibody is selected since it specifically binds to EGFR protein which is well-known to be overexpressed in cancer cells. ZnO nanorods/EGFR antibody probes bind to cancer cells through EGFR and emit purple light around 377nm as being excited by the UV luminance. By photoluminescent spectrum and image, cancer cells can be differentiated. The method prevents the photobleaching happened in traditional fluorescent detection and achieves the real-time detection. ZnO nanorods are also applied to IGZO-TFT protein biosensor to improve the sensitivity of detection. The bio-TFT is of extended gold pad to enlarge the sensing area and isolate the biochemical solutions. ZnO nanorods and EGFR antibody are used to functionalize the sensing pad for specific detection of EGFR. The biocompatibility and high surface-to-volume ratio of ZnO nanorods improve the sensitivity of the device significantly. By this novel structure, a time-saving and highly sensitive protein detecting process is developed. The bio-TFT is able to selectively detecting 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml.

並列關鍵字

cancer ； EGFR ； ZnO nanorods ； high sensitivity biosensor

參考文獻

[1]Chen, C. P., P. H. Lin, et al. (2010). "Investigation of light absorption properties and acceptance angles of nanopatterned GZO/a-Si/p(+)-Si photodiodes." Nanotechnology 21(21): 215201.

[2]Wei Wei, Guang-Hui Ma, Gang Hu, Di Yu, Tom Mcleish, Zhi-Guo Su and Zhe-Yu Shen. (2008). “Preparation of Hierarchical Hollow CaCO3 Particles and the Application as Anticancer Drug Carrier” J. AM. CHEM. SOC. (130): 15808–15810.

[3]Kim, D.-S., J.-E. Park, et al. (2006). "An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes." Sensors and Actuators B: Chemical 117(2): 488-494.

[4]Reyes, P. I., C.-J. Ku, et al. (2011). "ZnO thin film transistor immunosensor with high sensitivity and selectivity." Applied Physics Letters 98(17): 173702.

[5]B.K. Sohn, C.S. Kim. (1996). “A new pH-ISFET based dissolved oxygen sensor by employing electrolysis of oxygen.” Sens. Actuators B 34: 435–440.

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以氧化鋅奈米柱增進靈敏度及特異性之薄膜電晶體蛋白質檢測器

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