應用磁性奈米顆粒提昇金奈米狹縫電漿子共振訊號於
超高靈敏核酸分子標記及癌細胞的免標定偵測

Cancer is one of the leading causes of death in the developed world. Early detection and diagnosis of carcinomas has proven to be crucial in maximizing the efficacy of currently available therapy and in boosting the chances of patient’s survival. As such, a great deal of effort has been directed at developing methods for sensitive and rapid non-invasive detection of biomarkers at the early stage of the disease. This thesis focuses on developing a sensing platform for sensitive and label-free detection of cancer biomarkers. The method utilizes functionalized magnetic nanoparticles (MNPs) for signal enhancement in conjunction with surface plasmon resonance (SPR) on gold nanoslits. Chapter 1 provides background of biosensing technology, current biomarker detection method, an introduction of plasmonic methods and an overview of the gold Nanoslit SPR integrated with microfluidic as the sensing platform. Chapter 2 contains experimental procedure including gold nanoslit film fabrication, microfluidic fabrication and measurement setup. Chapter 3 demonstrates the application of our sensing platform to detect mRNA heterogeneous nuclear ribonucleoproteins (hnRNP B1) in two cancer cell lines, CL1–0 and CL1–5. Chapter 4 describes a novel microfluidic chip (funnel chip) integrated with SPR sensing platform for detection of rare biomarkers in large volume of sample. The application of this chip for a rare nucleic acid biomarker in urine of patient with kidney injury and rare cancer cells in human blood has been discussed. Chapter 5 summarizes the application of our sensing platform for biomarker detection.

並列關鍵字

SPR ； biomarker ； Label-Free Detection

參考文獻

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