應用金奈米粒子自組裝薄膜於奈米流道電動力濃縮之免疫分析電性量測

人體內微量的蛋白質配合奈微米尺度的流道設計，可利用電壓的施加使的微米流道內產生蛋白質濃縮的現象，藉由預濃縮能夠提升蛋白免疫檢測的靈敏度，應用於癌症的早期診斷，且可更快速的對人類血液進行分析，並減少生物樣本的消耗；利用設計的濃縮晶片能夠將樣本蛋白經濃縮後藉由濃度的提高而增加免疫反應中抗原抗體鍵結反應的靈敏度。本研究旨在研發並利用濃縮裝置使生物樣本液增強其免疫反應，流道母模採用具有良好生物相容性的PDMS轉印經由黃光微影製程的流道圖形，晶片部分則是使用APTES(3-Aminopropyl-triethoxysilane)在完成黃金電極圖形後的玻片上進行表面修飾，再將金奈米粒子自組裝於晶片表面，接著再將PDMS與晶片接合，然後施以高電壓形成電擊穿現象，藉由金奈米粒子自組裝薄膜可有效降低電擊穿所需之電壓，即完成奈米電動力濃縮裝置，晶片中同時製作金電極免疫反應區。本研究結果顯示，修飾於電極上的抗體可成功抓取經濃縮後的螢光胎牛血清蛋白(FITC-BSA)，且相較於未經濃縮之晶片，進行濃縮步驟後之免疫反應效果較佳。

關鍵字

集中排斥效應；奈米通道；電擊穿；預濃縮；免疫分析

並列摘要

The protein expression in human body can be used in the detection of cancer and other diseases in the early stage. Preconcentration is an important step that increases accuracy of subsequent detection, especially for samples with extremely low concentrations. Because of overlapping electrical double layers in the nanofluidic channel, the concentration polarization effect can be generated by applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, resulting in the fast accumulation of proteins in front of the ionic depletion zone, the so-called “exclusion-enrichment effect.” Proteins will accumulate by applying the electric voltage in the nano/microchannel. Due to the concentration-enhanced immunoassays and analyze rapidly to reduce consumption of the sample. The preconcentration nanofluidic chip for proteins was mainly fabricated by simple standard lithograph with replica of polydimethylsiloxane (PDMS). The proposed method for nanofissures formation utilizing the self-assembly of gold nanoparticle is aimed to assist electric breakdown. The experimental results indicated that antibody conjugated with proteins after preconcentrating. The results revealed that the impedance for the immunoassay after preconcentration increase significantly in this study.

並列關鍵字

immunoassay ； nanochannels ； concentration ； protein ； electric breakdown

參考文獻

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應用金奈米粒子自組裝薄膜於奈米流道電動力濃縮之免疫分析電性量測

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