應用奈米流道電動力濃縮蛋白質以增強免疫反應

對於有極低濃度的樣本，在檢測先進行預濃縮對於檢測的準確度是相當重要的一步。當我們施加電場在有電雙層重疊的奈米通道時，奈米通道的兩端會產生濃度極化的現象。因此，非線性電動流會使樣本蛋白在離子耗損區前快速的累積，即為排斥集中的現象。在本篇研究中，利用奈米裂縫的形成產生排斥集中現象預濃縮蛋白質。將蛋白質樣本通過電滲流驅動並積累在特定位置。本研究的目的是整合蛋白質濃度和免疫反應在同一片晶片上。蛋白質濃縮晶片是使用簡單的標準黃光微影製程與聚二甲基矽氧烷(PDMS)複製品製成。所使用的蛋白為胎牛血清白蛋白（BSA）和BSA抗體，利用抗體與抗原的免疫專一性，對本研究晶片進行驗證，本研究結果顯示，在免疫反應前進行預濃縮所有助於檢測，也驗證了該晶片的適用性。

關鍵字

蛋白質濃縮；自組裝；離子耗損區；排斥集中效應

並列摘要

Sample preconcentration is an important step that increases the accuracy of subsequent detection, especially for samples with extremely low concentrations. Due to the overlapping of electrical double layers in the nanofluidic channel, the concentration polarization effect can be generated by applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in the fast accumulation of proteins in front of the induced ionic depletion zone, the so-called exclusion-enrichment effect. Nanofractures were created in this work to preconcentrate proteins via the exclusion-enrichment effect. The protein sample was driven by electroosmotic flow and accumulated at a specific location. The purpose of this study is to integrate protein concentration and immunoassay in the microchip. The proposed chip for protein preconcentration was fabricated using simple standard soft lithography with a polydimethylsiloxane replica. An immunoassay of bovine serum albumin (BSA) and anti-BSA was carried out to demonstrate the applicability of the proposed chip

並列關鍵字

Preconcentration ； Protein ； Self-assembled ； Immunoassay

參考文獻

[2] Y. Gao, F. Y. H. Lin, G. Hu, P. M. Sherman, and D. Li, “Development of a novel electrokinetically driven microfluidic immunoassay for the detection of Helicobacter pylori,” Anal. Chim. Acta, vol. 543, no. 1–2, pp. 109–116, 2005.

[3] Y. Gao, P. M. Sherman, Y. Sun, and D. Li, “Multiplexed high-throughput electrokinetically-controlled immunoassay for the detection of specific bacterial antibodies in human serum,” Anal. Chim. Acta, vol. 606, no. 1, pp. 98–107, 2008.

[4] R. P. Sebra, K. S. Masters, C. Y. Cheung, C. N. Bowman, and K. S. Anseth, “Detection of antigens in biologically complex fluids with photografted whole antibodies,” Anal. Chem., vol. 78, no. 9, pp. 3144–3151, 2006.

[5] J. H. Lee, Y. A. Song, S. R. Tannenbaum, “Increase of Reaction Rate and Sensitivity of Low-Abundance Enzyme Assay using Micro/Nanofluidic Preconcentration Chip,” vol. 80, no. 164, pp. 3198–3204, 2008.

[6] H. L. Jeong, B. D. Cosgrove, D. A. Lauffenburger, and J. Han, “Microfluidic concentration-enhanced cellular kinase activity assay,” J. Am. Chem. Soc., vol. 131, no. 30, pp. 10340–10341, 2009.

國際替代計量

應用奈米流道電動力濃縮蛋白質以增強免疫反應

未授權

主題瀏覽