研發一免標定全血處理之微流道晶片進行血漿純化和白血球捕捉

Developing a label-free whole-blood processing microfluidic device for plasma collection and white blood cells trapping

蘇威宇(Wei-Yu Su)

指導教授 ： 黃念祖

國立臺灣大學/電機資訊學院/生醫電子與資訊學研究所/碩士(2017年)

https://doi.org/10.6342/NTU201704073

摘要

血液是現今最常被用來分析各式各樣人體訊息的生物樣本，其中常見的分析目標為紅血球、白血球以及血漿。為了要準確地得到這些訊息，必須將血液樣本中的各內容物進行前處理如：分離或稀釋。本論文設計一被動式分離全血微流道晶片，僅需使用少量的血液樣本(<10μL)，無需血球標記的前處理，同時進行全血的血漿分離純化(稀釋1.3至2.5倍)與紅、白血球的分離，並且30分鐘內以微柱陣列捕捉約3000顆白血球。目前此全血處理微流道晶片在分析目標上已有初步成果，且具有將全血細胞計數(Complete Blood Count, CBC)發展成實驗室晶片(Lab-on-a-Chip, LOC)之潛力。

關鍵字

全血 ； 微流道 ； 免標記 ； 細胞分離 ； 細胞捕捉

並列摘要

Nowadays, one of the most commonly used sample for health analysis is blood, of which red blood cells (RBCs), white blood cells (WBCs), and plasma are the most commonly used analytes. For direct analysis of blood, the samples should be pre-processed through separation or dilution. In this thesis, we designed a passive whole blood separation microfluidic device that can simultaneously separate plasma (with 2.2-2.5 times dilution) and WBCs from whole blood. The device can further trap about 3000 WBCs with micro-pillars in 30 minutes. The separation process is label-free and requires a low sample volume of 10 μL. The whole blood separation microfluidic device has the potential to achieve complete blood count (CBC) and develop into a Lab-on-a-Chip (LOC) device.

並列關鍵字

whole blood ； microfluidics ； label-free ； cells separation ； cells trapping

參考文獻

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