- 學位論文
具分泌木聚醣酶漂前黑液菌種形態鑑定及生化特性分析
Isolation and Biological Characteriistics of a Xylanase Generating Paenibacillus Strain from Black Liquor
摘要
木聚醣酶是能降解木聚醣的酵素，可與不同漂白程序結合，降低木質素含量、節省漂白及化學藥劑、增進漂白效果，並且只有極微量黏度改變及紙張性質變化與降低廢水AOX值。纖維素酶是能降解纖維素的酵素，存在少量可助紙漿漂白，並可運用於造紙廠纖維廢渣、分解黑液中殘餘的纖維等。本研究由台灣紙業股份有限公司新營廠漂前黑液中所採得、篩選出嗜熱性木聚醣降解菌株BL11，進行形態鑑定及生化特性分析，期能對本菌株有深入的了解，並對其生產之木聚醣酶及纖維素酶進行初步的探討，以利後續在製漿上應用的研究。 試驗結果顯示，BL11為paenibacillus sp.；革蘭氏陽性菌；具內孢子；最適生長條件：碳源為Maltose，氮源為Yeast extract，最佳生長溫度為60 ℃，最佳pH為5.5，catalase positive；oxidase negative；Indole negative；H2S negative；為好氧性菌株。 在基質利用方面，BL11能分解Glucose、L-Rhamnose、Mannose、Arabinose、Lactose、Xylan、Glycerol、D(+)-Maltose、Starch、D(+)-Raffinose、Avicel、CMC。 酵素活性試驗，菌株BL11在Basal medium中培養4-12小時所所分泌之CMCase、Avicelase、Salicinase及Xylanase酵素活性即可達最大值，但其中，CMCase活性在與其他文獻的比較下，其活性並不強，而另外三種酵素能在短時間內即達到最大值之酵素活性，因此有較佳的應用價值。 在未來的發展上，可以最佳生長條件培養後，大量生產酵素，以供未來深入的研究與應用。
關鍵字
木聚醣酶並列摘要
The xylanase is enzyme that degrades xylan. It can combine with different bleach procedures to reduce lignin quantity, to reduce bleach and chemicals, and to increase bleachability. Just with the slight change of viscosity and paper quality, the AOX was reduced. The cellulase is an enzyme of degrading cellulose. It is helpful to pulp bleaching, to decompose about paper mill fiber residue and remnant fiber of black liquid. The strain of thermophilic aerobe xylan-degrading bacteria was isolated from Taiwan Pulp & Paper Corporation Hsinying Mill’s black liquid before bleach in this study. Strain BL11 to proceed shape identification and biochemical characteristics was investigated in this study. Xylanase and cellulase of each isolates were further investigated. Strain BL11 was Paenibacillus sp., gram-positive rod bacterium, endospore-forming. The optimum growth conditions：The optimum growth carbon source and nitrogen source of strain BL11 was Maltose and Yeast extract, the optimum growth temperature was 60 ℃, the optimum pH was 5.5；catalase positive；oxidase negative；Indole negative；H2S negative, aerobic. About the different substrates used for utilization test, strain BL11could catabolize Glucose, L-Rhamnose, Mannose, Arabinose, Lactose, Xylan, Glycerol, D(+)-Maltose, Starch, D(+)-Raffinose, Avicel and CMC。 About the enzyme activity test, The CMCase, avicelase, salicinase and xylanase had the highest activity when the strain BL11 were incubated for 4-12 hours in the basal medium. But among them, the activation of CMCase is under comparing with other documents, its activation is not strong, but other three kinds of ferments can reach the ferment activation of the maximum promptly within short time , so there is better using value. In this study, BL11 had high-activity xylanase. When cultivated at the optimum growth conditions, the enzyme can be mass produced. For further investigation into the characteristics of thermophilic aerobe xylan-degrading bacteria.
並列關鍵字
Xylanase參考文獻
延伸閱讀
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