以孔洞澱粉進行微膠囊化影響Bifidobacterium longum B6其對模擬胃酸及膽鹽之敏感性與儲存安定性

Microencapsulation with porous starch carriers affects the storage stability and susceptibility of Bifidobacterium longum B6 to simulated gastric acid and bile salt

高淑惠(Shu-Hui Kao)

指導教授 ： 周正俊

共同指導教授 ： 呂廷璋(Ting-Jang Lu)

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.02488

摘要

微膠囊化是保護益生菌乾燥菌體的一種方式，為瞭解以孔洞狀澱粉顆粒為載體時是否可提高乾燥微膠囊化益生菌之存活率，本研究主要是利用10%不同水解率（30、45或60%）之孔洞澱粉及10%阿拉伯膠做為載體，藉由噴霧乾燥法、冷凍乾燥法進行Bifidobacterium longum B6之微膠囊化，並探討微膠囊化對B. longum B6在胃酸（pH 2.0）、膽鹽（2%）之耐受性及其貯存穩定性。
除未水解澱粉試驗組外，試驗前將澱粉顆粒以澱粉葡萄糖分解酵素預先水解為水解率約30、45與60%之三種孔洞狀澱粉試驗組，結果顯示，水解玉米澱粉1.5、2.5與4小時，可得到水解率30、45與60%孔洞澱粉。一般而言冷凍乾燥後B. longum B6之存活率高於噴霧乾燥者，以冷凍乾燥法利用水解率30及45%之孔洞澱粉為載體微膠囊化菌體之存活率分別為44.31及35.66%。在出口溫度為50℃下進行噴霧乾燥後，菌體存活率則分別為33.85及14.95%，將出口溫度提高至60℃時，造成菌體存活率之下降。微膠囊化粉末與pH 2.0胃酸溶液接觸3小時後B. longum B6之存活率皆比未微膠囊化之菌體高，呈現保護效果（p<0.05）。與2%膽鹽溶液接觸4小時後，顯示以水解率30與45%孔洞澱粉載體微膠囊化粉末中B. longum B6具有較高的存活率（p<0.05），且45%孔洞澱粉包覆者具有最佳之效果。以水解率30與45%孔洞澱粉為載體進行微膠囊化可提高B. longum B6菌體之儲存安定性，此外冷凍乾燥微膠囊中菌體之儲存安定性較噴霧乾燥者高，且於4℃儲存菌體之存活率較於25℃高，增加孔洞澱粉載體可小幅的延緩菌體儲存過程存活率下降之趨勢，顯示使用S30或S45為載體進行微膠囊化可提高益生菌在加工製程之存活率。

關鍵字

孔洞澱粉 ； 雙叉桿菌 ； 微膠囊化 ； 乾燥 ； 存活

並列摘要

Microencapsulation is a method to protect dry probiotic powders. To understand if the survival rate of microencapsulated probiotic could be raised with the porous starch granules are taken as the carriers. In this study, using 10% porous starch obtained at different hydrolysis rate (30, 45, and 60%) and 10% gum arabic as the carriers, the microencapsulated cells of Bifidobacterium longum B6 were first prepared by spray-drying or freeze-drying. Survival of these microencapsulated and free cells of test organism in simulated gastric juice (pH 2.0) and bile solution (2%) as well as their stability during storage were examined.
In addition to the treatment group of native starch, we firstly hydrolyzed the corn starch granules with amyloglucosidase into three porous starch treatment groups: the one with hydrolysis rate of about 30, 45, and 60%. It was found that the porous starch with hydrolysis rate 30, 45, and 60% （porous starch-30, 45 and 60%） could be derived from native starch degraded for 1.5, 2.5, and 4 h, respectively. Survival of B. longum B6 is generally higher after freeze-drying than spray-drying. Freeze-drying with porous starch-30% + gum arabic or porous starch-45% + gum arabic. B. longum B6 showed a survival rate of 44.31 and 35.66%, respectively. While spray-drying with an outlet-air temperature of 50℃, B. longum B6 exhibited a survival rate of 33.85 and 14.95%, respectively. Elevation of outlet-air temperature resulted in reduced survival of treat organism. Regardless of drying method, B. longum B6 encapsulated with gum arabic alone or gum arabic + porous starch-30 or 45% showed a higher survival in the simulated gastric juice (pH 2.0) than the nonencapsulated cells. Encapsulated with porous starch-30 or 45% + gum arabic also exhibited a protective effect on test organism in 2% bile solution. An enhanced storage stability were also noted with these microencapsulated B. longum B6. Besides, survival of the test organism encapsulated with freeze-drying and held at 4℃ is higher than that encapsulated with spray-drying and stored at 25℃. Increasing porous starch carriers could slightly delay the decrease of the survival rate of bifidobacteria in the storage process. It revealed that using S30 or S45 as the carriers to microencapsulated probiotic could enhance the survival rate of probiotic in the processing.

並列關鍵字

porous starch ； Bifidobacterium ； microencapsulation ； drying ； survival

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