- 學位論文
多光子偏振及廣義偏振顯微術於皮膚影像之應用
Applying two-photon polarization and generalized polarization imaging to skin structure
摘要
通過皮膚藥物的傳遞(transdermal drug delivery)是利用物理及化學方法將藥物傳送通過皮膚的一種機制，它具有諸多優點包含了可維持血液中藥物濃度的穩定、無痛、及方便性。然而，有效的將藥物傳遞通過皮膚卻十分困難，因為角質層扮演一個阻擋外來物如藥物進入的屏障，有釵h研究針對利用各種物理及化學機制增加藥物傳遞的速率，如化學增進劑、微小電流、以及大電流、光—力學波以及超音波，然而這些方法的作用機制仍然沒有全然的被了解。這個實驗中我們應用雙光子偏振及廣意義偏振影像來了解人類皮膚角質層以及真皮纖維的結構，我們發現雙光子偏振影像是一個很好的方法來研究皮膚結構的排列，而廣義偏振則可用於了解皮膚的微觀化學環境，我們證明結合偏振及廣義偏振影像可以了解油酸於通過皮膚藥物的傳遞的作用機制，我們的結果說明結合偏振及廣義偏振影像可使我們監控生物結構中物理及化學環境的變化。
並列摘要
Transdermal drug delivery utilizes physical or chemical techniques to transport drugs across the skin. This approach has many advantages including being able to maintain a steady drug concentration in vivo, painless, and convenient. However, to efficiently delivery effective dosage of therapeutic through the skin is difficult due to the presence of the stratum corneum as the main barrier to the ingress of exogenous materials, such as therapeutic drugs. Many investigations have been performed to improve drug permeation across the skin using chemical or physical enhancement techniques: application of chemical enhancers, iontophoresis, electroporation, photomechanical wave and sonophoresis . However, the mechanisms of these techniques are not perfectly understood. In this work we demonstrate the application of multiphoton polarization and generalized polarization imaging in resolving the structures in surface stratum corneum and dermal layers in ex-vivo human skin. We found that multiphoton polarization imaging can be a powerful method in identifying structural orientations in the skin. On the other hand, multiphoton generalized polarization imaging is useful in revealing the microscopic chemical environment of the skin. We demonstrate that by combing mutiphoton polarization imaging and generalized polarization imaging, we can gain insight into the mechanism of the oleic acid enhanced transdermal drug delivery. Our results show that combined polarization and GP microscopy can be used to characterize the physical and chemical changes in biological structures.