介電泳應用於血漿/紅血球分離及紅血球操控之研究

Dielectrophoresis on Plasma/RBC Separation and RBC Manipulation

羅英傑(Ying Jie Lo)

指導教授 ： 胡文聰

國立臺灣大學/工學院/應用力學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01627

摘要

本論文利用介電物質材料特性在交流電場下所形成介電泳力，作為血球血漿分離及血球操控運用。介電泳基礎在於粒子與溶劑之間，介電能力及導電能力的差異性誘導出一個決定於頻率高低、電場空間分佈及相位空間分佈且具方向性之力量。
透過微機電曝光、顯影等製程達到元件微小化及提高介電泳力量達到影響粒子運動，並且透過基本的數值分析計算求出電場分佈及血球軌跡以作為元件設計的依據。利用PDMS高生物親和力材料作為流道製程之材料以便未來的整合。
設計出之血球血漿分離方式有階梯型、傾斜型、梯度分佈型電極與三維流道輔助等，而在粒子操控方面旅波電泳於血球之利用、血球集中元件、血球分配元件均試驗成功。

關鍵字

介電泳 ； 血球

並列摘要

This thesis takes advantage of dielectric property of material, where a dielectrophoretic (DEP) force is induced in a sinusoidally time-variant electric field to achieve cell-plasma separation and cell manipulation. Dielectrophoretic theory is based on the distinct dielectric and conductive properties of cell and medium. This distinction will physically induce a directional force depending on frequency, spatial electric strength, and spatial electric phase distribution.
Through fabrication of MEMS, devices miniaturized are to increase the influence of DEP force on separation and manipulation of cells. Additionally, with the aid of numerical simulation of electric field and cell trajectory, more effective devices are designed. The use of bio-compatible material polydimethysilloxane (PDMS) proved ease of fabrication and integration [1].
Types of cell-plasma separator tested various electrode design include stair, inclined, and gradient confuguration, and 3D channel assisting design. For cell manipulation, traveling wave, cell concentrator, and cell portioning devices are all tested and their performance quantified.
Results show successful separation of red blood cell (RBC) and plasma vis DEP. for a wide range of electrode geometry configurations. Traveling wave DEP, however, was more difficult to implement. Manipulation of RBC proved viable using the non-uniform E-field at the tip of multi-electrode design.

並列關鍵字

blood cell ； dielectrophoresis

參考文獻

[1] George M. Whitesides, “Emanuele Ostuni, Shuichi Takayama, Xingyu Jiang, and Donald E. Ingber, “Soft Lithography in Biology and Biochemistry,” Annu. Rev. Biomed. Eng. 2001. 3:335-73

[6] Michael P. Hughes and Hywel Morgan, “Measurement of bacterial flagellar thrust by negative dielectrophoresis,” Bioelectronics Research Centre, Unversity of Glasgow, Biotechnol, Prog, 1999.

[9] Machael Pycraft Hughes, “Nanoelectromechanics in Engineering and Biology,” CRC PRESS, 2003.

[11] David J. Griffiths, “Introduction to Electrodynamics,” Prentice Hall, 1999.

[2] Marc J. Madou, “mentals of microfabrication,’’ CRC press 1997