介電泳細胞分離與輸送的研究

Study of the Cell Separation and Delivery Using Dielectrophoresis

馮德威(Tak-Wai Fung)

指導教授 ： 李雨

國立臺灣大學/工學院/應用力學研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.03205

摘要

本論文利用微機電技術製造旅波式介電泳幫浦及細胞分離晶片。旅波式介電泳幫浦為一截面為矩形的流道，在其中一壁面鋪上如鐵軌般垂直於流道的電極，並施以六組四相位差的交流訊號，此時紅血球將會被傳統式介電泳力抬離電極表面，旅波式介電泳力則會驅動紅血球往相位遞增的方向前進，血漿則會藉由紅血球移動時的黏滯阻力被帶動，而達到傳輸血液的效果；細胞分離晶片亦使用旅波式介電泳力，當細胞於同一位置出發，經過與流道具有特定傾斜角度的電極陣列，會產生不同幅度的偏向，即可分辨出不同的細胞。本文針對這兩部份的研究，完成的工作如下：(1)找出最佳的旅波式介電泳幫浦操作頻率，5MHz時全血平均流速可達50μm/s。(2)比較在首根電極前增加兩根輔助電極於不同情況下是否能提升幫浦的效能，結果主要顯示輔助電極可助縮短幫浦達穩態運輸時的暫態時間，如10MHz時由30分鐘縮短為20分鐘。(3)探討了血液濃度對旅波式介電泳幫浦的影響。(4)測試幾種不同的流道設計，電極區漸縮的流道有較好的表現，幫浦平均流速從原本的11.1μm/s提升至16.5μm/s。(5)本研究設計的細胞分離晶片，可在10MHz、背景流速69.44~148.81μm/s時，將RPMI培養液中的CL1-0、CL1-5和白血球分辨出來；在10MHz、背景流速49.60~128.97μm/s時，將PBS緩衝液中的Jurkat、白血球和紅血球分辨出來。

關鍵字

旅波式介電泳幫浦 ； 輔助電極 ； 細胞分離

並列摘要

MEMS techniques are emploged to fabricate travelling wave dielectrophoretic (TWDEP) pumps and cell separation chips. The pump is a micro channel with a rectangular cross-section with an electrode array on one of its walls. The electrodes are operated under ac signal 90º shift of between neighboring electrodes, and there are 24 basic electrodes on an array. The negative dielectrophoretic force repels the red blood cells from the electrode surface and the travelling wave dielectrophoretic force drives the cells along the direction of increasing phase. The plasma will be drag through viscous force as the red blood cells move, and the whole blood is delivered. In cell separation chips, the paths of different cells in a background flow are deflected by a designed travelling wave dielectrophoresis, and thus different cells are separated. Several works have been completed: (1) Optimum frequency for whole blood delivery in travelling wave dielectrophoretic pumps is found to be around 5MHz. (2) Two assistant electrodes before the basic electrode array can shorten the transient time of the TWDEP pump, and can enhance pump performance in some cases. (3) Performance of the TWDEP pump at different volume ratios of cells have been studied. (4) Different channel designs were carried out, and it is found that electrode array in converging channel section has better pump performance, the average velocity increases from 11.1μm/s promote to the 16.5μm/s. (5) At 10MHz and background velocity 69.44~148.81μm/s , the cell separation chip can separate CL1-0, CL1-5 and WBC in the RPMI medium ; At and background velocity 49.60~128.97μm/s, our cell separate chip can recognize Jurkat, WBC and RBC in the PBS solution.

並列關鍵字

Travelling wave dielectrophoretic pump ； Assistant electrode ； Cell separation

參考文獻

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