豬隻脂肪細胞的基因表現

The expression of genes related to adipocytes in pigs

王泓洲(Hung-Chou Wang)

指導教授 ： 丁詩同

國立臺灣大學/生物資源暨農學院/動物科學技術學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02633

摘要

無資料

關鍵字

脂肪細胞 ； 脂肪前身細胞 ； 抑制性雜合扣除法 ； 基因 ； 豬

並列摘要

The purpose of this study was to detect differential expression of genes related to adipocytes in pigs by suppression subtractive hybridization (SSH). Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. The cDNA from preadipocytes was subtracted from the cDNA from adipocytes. The subtracted gene fragments were cloned into pGEM-T Easy TA cloning vector. We selected 384 clones for gene sequence determination and for further analysis. These genes were subjected to a differential screening procedure to confirm the differential expression of genes between the two cell types in pigs. We found that at least 36 genes were highly expressed in the adipocytes compared with preadipocytes. Among these, six genes including two novel genes with the greatest differences were selected and confirmed by Northern analysis. We found that angiotensin I-converting enzyme (ACE), Ataxia-telangiectasia mutated protein (ATM), calpain1, and stearoyl coenzyme A desaturase 1 (SCD1) were highly expressed in adipocytes compared with preadipocytes (P < 0.05). The relative mRNA abundance of ACE, ATM, calpain1, SCD1, and two novel genes discovered in the current study was increased at the later stages of adipocyte differentiation (P < 0.05). The results confirmed that the genes involved in lipid metabolism and adipocyte differentiation were highly expressed in porcine adipocytes. However, further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.

並列關鍵字

adipocyte ； gene ； preadipocyte ； procine ； suppression subtraction hybridization

參考文獻

Acharya, K. R., E. D. Sturrock, J. F. Riordan, and M. R. Ehlers. 2003. Ace revisited: A new target for structure-based drug design. Nat Rev Drug Discov 2: 891-902.

Ailhaud, G., M. Teboul, and F. Massiera. 2002. Angiotensinogen, adipocyte differentiation and fat mass enlargement. Curr Opin Clin Nutr Metab Care 5: 385-389.

Altiok, S., M. Xu, and B. M. Spiegelman. 1997. Ppargamma induces cell cycle withdrawal: Inhibition of e2f/dp DNA-binding activity via down-regulation of pp2a. Genes Dev 11: 1987-1998.

Aranda, A., and A. Pascual. 2001. Nuclear hormone receptors and gene expression. Physiol Rev 81: 1269-1304.

Barak, Y., M. C. Nelson, E. S. Ong, Y. Z. Jones, P. Ruiz-Lozano, K. R. Chien et al. 1999. Ppar gamma is required for placental, cardiac, and adipose tissue development. Mol Cell 4: 585-595.