連接子及界面條件對石英振盪微天平在水浴量測之研究

Study of Linker & Interfacial Effect on the Quartz Crystal Microbalance in Aqueous Condition

吳爵宇(Chueh-Yu Wu)

指導教授 ： 李世光

共同指導教授 ： 林世明(Shiming Lin)

國立臺灣大學/工學院/應用力學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.02810

摘要

質量吸附造成頻率下降為石英振盪微天平在生物應用上的一般概念，但尿素解構作用和殺鼠靈的鍵結反應在頻率反應上得異常現象已經無法用一般概念解釋，在將質量和黏彈性質改變造成的影響去除後，此類現象將被歸類為界面現象之影響。論文中嘗試以多變數來偵測和分析此現象的影響程度，而方法便是建立一多諧振探測之石英振盪微天平，而實驗結果顯示尿素人類血清白蛋白的解構使得單純由表面親疏液體性質變化對石英振盪微天平的影響被觀察到。
　於是整合系統之多諧振探測之石英振盪微天平提供了多參數量測，這使得一般帶有質量、黏彈性質、表面性質變化的生物反應可以被描述和分析，同時，自行組裝的系統具有研究進階表面現象的潛能，成為未來藥物開發的有利工具。

關鍵字

生醫檢測 ； 石英 ； 阻抗分析 ； 血清白蛋白 ； 尿素 ； 蛋白質 ； 解構

並列摘要

Traditional prediction about frequency reduction of Quartz Crystal Microbalance (QCM) after materials bonding was challenged by the phenomenon of warfarin binding and denature by urea. The response was classified as the interfacial effect after eliminating the mass and the viscoelasticity effects. Multi-parameter detection that includes the mass, the viscoelastic effect, etc. was detected and analyzed by using high-overtone Quartz Crystal Microbalance (HQCM) in this thesis. Furthermore, QCM was for the first time to make the observation of the pure transition of hydrophilic to hydrophobic property possible during the case of urea unfolding.
HQCM was fabricated and integrated into a complete system that enables multiple parameters measurement. The general biological interaction inducing mass, viscoelastic, interfacial effect will be more apparently described and analyzed on the platform of HQCM. At the same time, the lab-built system provides much freedom, having potential to develop advanced study of interface science. The whole system can potentially find application in drug discovery.

並列關鍵字

biosensor ； quartz ； impedance analysis ； human serum albumin ； urea ； protein ； unfolding ； denature

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