海生物腐蝕抑制材料性能快速檢測技術之開發

The development of rapid test for inhibiting marine microbiologically influenced corrosion material

陳穎(Ying Chen)

指導教授 ： 李岳聯

國立臺灣大學/工學院/工程科學及海洋工程學研究所/碩士(2021年)

https://doi.org/10.6342/NTU202104330

摘要

海洋生物污損是影響海洋工業的世界性問題，像是在長時間航行的船壁上常常會發現大量生物附著，這不但會使船隻航行時阻力增加，並且提升耗油量，更嚴重還會導致微生物腐蝕的發生，故近年全球都試圖研發新型殺菌劑塗層來抑制生物污損。由於過去檢測塗層效果都需於海洋中長時間實地檢測，本文擬提出一系列快速檢測抑制微生物之方法，不管任何殺菌劑都能透過96孔盤棋盤稀釋法及瓊脂稀釋塗盤法，在短時間內得到其最小抑制濃度(MIC)及最小殺菌濃度(MBC)。當中會選用不同種類的殺菌劑來證明此方法的準確性，例如金屬銀離子、常見商用殺菌劑百里酚(Thymol)及十二烷基二甲基苄基氯化銨(BDMDAC)，還有複合殺菌劑組。並且利用結果之有效抑制濃度的倍數來製作殺菌劑塗層，藉由靜態浸泡14天後拍攝塗層試片倒立共軛焦雷射掃描顯微鏡(CLSM)的結果及動態沖蝕試驗後的結果，能說明在依照其最佳濃度的倍數來製作新型防污塗層時，能快速且準確地找出最有效之最小抑菌塗層濃度，並有效降低對於環境的汙染，達到永續之目標。

關鍵字

快速檢測方法 ； 最小抑制濃度 ； 最小殺菌濃度 ； 倒立共軛焦雷射掃描顯微鏡 ； 動態沖蝕試驗

並列摘要

Marine biofouling is a worldwide problem affecting maritime industries. For example, large numbers of organisms are often found on the ship hull after sailing in several years, these would not only increase frictional drag, lead to increase fuel consumption, more seriously, it would cause microbiologically influenced corrosion. Therefore, lots of anti-fouling coatings have been developed in recent years. In the past, researcher testing the effect of anti-fouling coatings required long-term field test in the ocean. This study intends to publish a method to quickly test the minimum concentration for inhibited microorganisms. In every kind of biocides, it can quickly get the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) through 96-well plate microdilution chequerboard method and agar dilution method. In this article, different types of biocides are used to confirm the usability of this method, such as metallic silver ions (Ag+), commercial biocides Thymol and the Benzyldimethyldodecyl ammonium Chloride (BDMDAC), and composite biocides. To further confirm the effectiveness of the minimum concentration determined by this rapid test, the specimen with biocide coating according to the multiple of the rapid test method result on the silver ions, and the confocal laser scanning microscopy (CLSM) is conducted on the specimen after static immersion and dynamic erosion test for 14 days. The result shows that this method can quickly and accurately find the most effective minimum antibacterial coating concentration, and can also reduce the pollution to the environment.

並列關鍵字

rapid test method ； minimum inhibitory concentration ； minimum bactericidal concentration ； confocal laser scanning microscopy ； dynamic erosion test

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