飼糧中添加抗生素對肉雞生長性狀、腸道免疫生理功能及菌相之影響

Effect of Antibiotics on the Growth Performance, Intestinal Immunophysiology and Gut Floral Composition in Broilers

洪靖崎(Ching-Chi Hung)

指導教授 ： 陳保基

共同指導教授 ： 余佳慧

國立臺灣大學/生物資源暨農學院/動物科學技術學研究所/碩士(2008年)

https://doi.org/10.6342/NTU.2008.02702

摘要

本試驗探討飼糧中添加抗生素對肉雞生長性狀、腸道生理免疫功能及菌相之影響。選取192隻1日齡愛拔益加雛雞，隨機分置對照組 (control)、可利斯汀 (colistin) 20 ppm、泰黴素 (tylosin) 55 ppm及氯四環黴素 (chlortetracycline) 55 ppm四組。試驗進行2批次，每批次96隻，每個處理組為兩重複，每重複12隻，分別於0、3週及6週時，進行腸道生理、菌相及免疫能力測定。結果顯示，於6週齡時，飼糧中添加可利斯汀和泰黴素兩組雞隻之體重及採食量顯著高於對照組，添加氯四環黴素組則與對照組無顯著差異，且添加可利斯汀和泰黴素會提高空腸的蔗糖酶 (sucrase) 及麥芽醣酶 (maltase) 的活性 (P<0.05)。在腸道電生理方面，添加可利斯汀組腸道中的離子電流 (short-circuit current) 及電導係數 (conductance) 顯著高於對照組 (P<0.05)，但於泰黴素及氯四環黴素則無顯著影響 (P>0.05)。在腸道組織形態方面，於3週齡時，添加可利斯汀會促進空腸及迴腸絨毛表面積，泰黴素則對迴腸形態無影響，但是會減少空腸的黏膜肌層厚度及腺窩深度，而添加氯四環黴素，則會增加空腸及迴腸的黏膜肌層厚度。在菌相方面，在0、3及6週，對照組雞隻肝臟皆有細菌的存在，添加抗生素組能顯著減少於3週齡雞隻肝臟及脾臟中的細菌數量 (P<0.05)，又可利斯汀及泰黴素添加組於6週齡時，顯著減少腸道中大腸桿菌的數量 (P<0.05)。在免疫方面，添加抗生素對於脾臟淋巴球增生及腸道中骨髓過氧化酵素活性 (myeloperoxidase activity) 並無影響 (P>0.05)，但所有抗生素處理組於3週齡時，均能發現血液中免疫球蛋白G (immunoglobulin G, IgG) 含量增加 (P<0.05)。泰黴素添加組於6週齡時則顯著減少血液免疫球蛋白A (IgA) 及腸道黏膜上之分泌型免疫球蛋白A的含量 (P<0.05)，可利斯汀同樣減少血液中免疫球蛋白A，而氯四環黴素處理組則無顯著影響。在抗體力價方面，泰黴素添加組會顯著增加白肉雞對傳染性支氣管炎 (Infectious Bronchitis) 之抗體力價 (P<0.05)，可利斯汀與氯四環黴素則無顯著影響。腸道免疫方面，泰黴素於6週齡時會促進迴腸上皮內T細胞受器γδ (TCR γδ) 總量的增加，而氯四環黴素處理組則會增加腸道中溶菌酶酵素 (lysozyme) 的含量 (P<0.05)，但可利斯汀處理則無顯著影響。綜言之，飼糧中添加可利斯汀及泰黴素可能藉由增加採食量，促進腸道黏膜雙醣酶的活性，改變腸道的形態，並減少腸道中的大腸桿菌及減少細菌轉移至肝臟及脾臟，而促進肉雞生長。

關鍵字

抗生素 ； 腸道生理 ； 免疫

並列摘要

The use of antibiotics as a growth promoter will be eliminated in the near future. In order to assess the impact of antibiotic substitutes on intestinal fuctions, the basic and antibiotic-induced changes on growth, intestinal immunophysiology and gut flora composition were investigated in broiler chickens. One hundard ninety two broilers were randomly allocated to 4 treatments with 4 replicates of 12 birds eachfor treatment. The 4 treatments were: control, 20 ppm colistin, 55 ppm tylosin and 55 ppm chlortetracycline supplementation. The intestinal physiology, gut flora composition in the intestine and immune function were measured at 0, 3 and 6 weeks of age. Results showed that the body weight gain and feed efficiency were significantly increased in colistin and tylosin supplementation group, but were not in chlortetracycline group at 6 weeks of age when compared with control (P<0.05). The short-circuit current and conductance of ileum in colistin group were greater than control (P<0.05). Gut mucosal maltase and sucrase activities were increased (P<0.05) in the colistin and tylosin groups at 6 weeks of age. In intestinal morphology, the colistin supplemented group had increased villus area in jejunum and ileum. The tylosin group showed decreased musculars mucosal thickness and crypt depth in jejunum but not in ileum (P<0.05). However, the addition of chlortetracycline increased the thickness of muscular mucosa in jejunum and ileum compared with control (P<0.05). In the aspect of gut flora composition, the coliform counts were also decreased in the intestine of colistin and tylosin supplementation groups at 6 weeks of age, but no significant difference was seen in chlortetracycline when compared to control. Enteric bacteria were present in liver of control chickens during experimental period. All antibiotics supplementation significantly inhibited the phemomena of bacteria translocated to the liver and spleen at 3 weeks of age. For immune responses, there were no significant difference in splenocytes proliferation and myeloperoxidase activity among treatments. In the aspect of humoral immune fuction, the antibiotic groups all displayed increased IgG antibody in blood when compared with control group (P<0.05). The tylosin supplemented group had increased antibody titer against Infectious bronchitis at 3 weeks of age and enhanced expression of T cell receptor γδ at 6 weeks of age, but decreased blood IgA levels and secretory IgA in the mucosa of jejunum (P<0.05). The chlortetracycline supplementation improved intestinal innate immunity by increased mucosal antimicrobial lysozyme level (P<0.05). In conclusion, the growth enhancement and increased feed intake by colistin and tylosin antibiotic supplementations were possibly due to reduce numbers of gut pathogenic bacteria and less translocation to intestinal organs, increase gut disaccharidase activities and augmentation of IgG antibody levels.

並列關鍵字

Antibiotics ； Intestinal physiology ； Immunity

參考文獻

林丹卉、王惟芬譯。抗生素的迷思：濫用抗生素對醫療的影響。2004。城邦文化事業股份有限公司。

Aksoyek, S., I. Cinel, D. Avlan, L. Cinel, C. Ozturk, P. Gurbuz, A. Nayci, and U. Oral. 2002. Intestinal ischemic preconditioning protects the intestine and reduces bacterial translocation. Shock. 18: 476-480.

Al-Ankari, A. S., and A. M. Homeida. 1996. Effect of antibacterial growth promoters on the immune system of broiler chicks. Vet. Immunol. Immunopathol. 53: 277-283.

Al-Batshan, H. A., J. L. Sell, J. Piquer, E. Mallarino, M. F. Soto-Salanova, and C. R. Angel. 1992. Responses of turkey poults to virginiamycin as influenced by litter condition and experimentally induced stunting syndrome. Poult. Sci. 71: 894-904.

Alcalde, A. I., Y. Barcina, A. Ilundain, and J. Larralde. 1987. Effect of amoxicillin on galactose transport across rat small intestine. Drug Nutr. Interact. 5: 71-79.