膠原蛋白結構與組成對於骨母細胞貼附、生長與功能的影響

Effect of Collagen Conformation and Components on Adhesion, Growth and Functions of Osteoblast-Like Cells

鄭育慧(Yu-Hui Cheng)

指導教授 ： 蔡偉博

國立臺灣大學/工學院/化學工程學研究所/碩士(2005年)

https://doi.org/10.6342/NTU.2005.02954

摘要

膠原蛋白（Collagen），是多細胞動物體內含量最多的蛋白質，幾乎存在於所有組織中。膠原蛋白被廣泛應用在醫學上之生醫材料，如組織生長、燒燙傷敷料、傷口癒合等。本研究的目標在於探討膠原蛋白的型態，和glycosaminoglycan（GAG）對於骨母細胞貼附、生長與功能的影響。我們在tissue culture polystyrene（TCPS）表面上，塗佈膠原蛋白（soluble collagen）、纖維狀膠原蛋白（fibrous collagen）以及在高溫下變性膠原蛋白（denatured collagen），再將骨母細胞接種在上面，觀察細胞在不同表面上的貼附量、細胞型態、生長情況，以及功能表現。我們發現，塗佈上soluble collagen與fibrous collagen的表面，對於細胞貼附及生長效果比denatured collagen表面好，同時其鹼性磷酸酶（Alkaline Phosphatase）的活性也比較大。此外，我們也發現將膠原蛋白與肝素（heparin）之混合液塗佈在TCPS上，當肝素濃度為0.02 mg / mL時，對於細胞貼附的促進效果最佳，並且混合液會隨著肝素濃度的增加，細胞數有減少的趨勢。

關鍵字

膠原蛋白 ； 骨母細胞 ； 細胞貼附 ； 鹼性磷酸酶肝素

並列摘要

Collagen and glycosaminoglycans are the major components of extracellular matrix, and are important in tissue formation and tissue engineering. This study was proposed to investigate the effects of conformation of type I collagen, and the effects of glycosaminoglycan on adhesion, proliferation and function of human osteosarcoma MG-63. Cells were seeded on TCPS plates coated with soluble collagen, fibrous collagen and heat-denatured collagen, or mixture of collagen and heparin. The attachment, morphology, growth and functions of cells were examined. We found that soluble collagen and fibrous collagen promoted cell attachment and growth more than denatured collagen. Cells cultured on soluble collagen coated plate had higher alkaline phosphatase activity than on the other substrates. In addition, we found that the plate coated with mixture of 0.02 mg/mL heparin and collagen supported better cell adhesion than the substrates coated with higher heparin concentration.

並列關鍵字

Collagen ； osteoblasts ； cell adhesion ； Alkaline Phosphatase ； heparin

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