The contents of this article chiefly explain the effects on grinding Ti-6A1-4V when using nano-fluid and the general water-based fluid. Assisting this effect by using the production of MQL and the cutting fluid method to analyze and compare. The contents analyze and inspect the differences when using nano- fluid, water-based fluid and MQL through the usage of wheel leading and wear, grinding force, surface of workpiece and roughness, also to analyze the diversification. The conclusion of this experiment is that MQL is the most effective to reduce wheel leading, and is most prone to achieve better surface of workpiece and roughness than the general wet grinding. Different kinds of cutting fluid also use the method of MQL in order to most effectively reduce roughness. When using MQL, we acquire a flatter workpiece in order that we could economize the finished cost and to enhance efficiency.