攝食雙叉桿菌鮮乳對腸道菌相與Caco-2及HT-29毒性之影響

Effects of fresh milk plus Bifido® intake on the intestinal microflora and cytotoxicity on Caco-2 and HT-29

邱佩娟(Pei-Jiuan Chyou)

指導教授 ： 游若篍

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.03229

摘要

摘要
人體腸道菌相十分複雜，其中有益菌雙叉桿菌(bifidobacteria)與有害菌產氣莢膜梭菌(Clostridium perfringens)菌數的消長，與人體健康關係十分密切。本試驗探討攝食雙叉桿菌鮮乳在四週的試驗期間對24位健康成人腸道菌相、糞便水份含量、pH值及其萃取液對細胞毒性之影響。試驗分成四個時期，第一期為飲食調整期，第二、三期為攝食雙叉桿菌鮮乳的前期與後期，第四期為排空期。另外再進行二十天之雙叉桿菌鮮乳儲存安定性試驗，探討在一般的儲存溫度與運送過程溫度變動下，對雙叉桿菌鮮乳品質的影響。
結果顯示，攝食雙叉桿菌鮮乳可使受試者腸道中雙叉桿菌顯著增加(p < 0.05)；而腸道有害菌產氣莢膜梭菌顯著減少(p < 0.05)。試驗期間受試者糞便檢體的水分含量、pH值無統計上的差異，且受試者的身高、體重、BMI值及血液生化值均無顯著變化，皆在標準範圍內(p > 0.05)。
接著以MTT細胞存活分析法，測定糞便檢體萃取液對腸道細胞株Caco-2及HT-29之細胞毒性。結果顯示，低濃度之糞便檢體萃取液對腸道細胞不具毒性，在雙叉桿菌鮮乳攝食期間，可顯著降低高濃度糞便檢體萃取液對腸道細胞的毒性(p < 0.05)。顯示攝食雙叉桿菌鮮乳，不會對受試者生理上造成不良的影響。可有效增加腸道益菌數，減少害菌數，並降低糞便中毒性物質對腸道細胞的傷害，具改善腸道健康之功能。
雙叉桿菌鮮乳儲存安定性試驗結果顯示，含菌量6.63 log CFU/mL之雙叉桿菌鮮乳，在一般鮮乳十天之保存期限內，儲存溫度不論是在4℃或11℃，其雙叉桿菌活菌數皆大於6.2 log CFU/mL，pH值維持在6.7-6.8，可滴定酸度為0.1%，皆正常範圍之內，在保存期間生化特性穩定，品質安定。

關鍵字

腸道菌相 ； 雙叉桿菌 ； 產氣莢膜梭菌 ； 細胞毒性 ； 存活率

並列摘要

Abstract
The human gastrointestinal is a complex ecosystem, the balance of beneficial bacteria bifidobacteria and harmful Clostridium perfringens is highly related to human health. This study was conducted to investigate the effect of intake fresh milk plus Bifido® on the intestinal microflora, fecal water content, pH values and fecal extracts cytotoxicity on Caco-2 and HT-29 in 24 healthy adults. Furthermore, the changes of viable bifidobacteria, pH value and titratable acidity in fresh milk plus Bifido® store at 4℃ and 11℃ for 20 days were also studied.
Results indicated that intake the fresh milk plus Bifido® significantly increased the colony counts of fecal bifidobacteria (p < 0.05), decreased the growth of C. perfringens (p < 0.05) and significantly increased the log number ratio of bifidobacteria to C. perfringens (p < 0.05). Subjects’ height, weight, BMI, blood parameters, fecal water content and fecal pH had no apparent changes during experimental period. Each period of subjects’ fecal extracts at low concentrations were no cytotoxicity on Caco-2 and HT-29. The cytotoxicity of subjects’ fecal extracts at high concentrations on Caco-2 and HT-29 were significantly reduced during fresh milk plus Bifido® intake periods (p < 0.05). These results suggested that intake the fresh milk plus Bifido® were beneficial in improving the intestinal flora and decreasing cytotoxic materials in the intestinal tract of health adults.
Fresh milk plus Bifido® contains 6.63 log CFU/mL bifidobacteria. The survival number of bifidobacteria in fresh milk plus Bifido® was maintained at 6.2 log CFU/mL during 10 days of shelf life time. The changes of pH value and titratable acidity in fresh milk plus Bifido® did not change statistically during the experimental period (p > 0.05).

並列關鍵字

Intestinal flora ； bifidobacteria ； Clostridium perfringens ； Cytotoxicity ； Viability

參考文獻

紀玉霜：不同工作週期之低能量超音波刺激在體外對纖維母細胞的影響。私立中原大學醫學工程學系碩士論文(2003)。

翁麗華：攝食複合乳酸菌粉及優酪乳對健康成人腸道菌相及致突變性之影響。國立臺灣大學食品科技研究所碩士論文(2006)。

林國暉：乳酸發酵蔬菜萃取物對4-nitroquinoline N-oxide基因毒性及數株癌細胞抑制之研究。國立臺灣大學食品科技研究所碩士論文(2006)。

鄭妃彣：薏苡麩皮甲醇萃取物之細胞毒性成分分離與純化。國立臺灣大學食品科技研究所碩士論文(2006)。

Akalin AS, Fenderya S, Akbulut N. 2004. Viability and activity of bifidobacteria in yogurt containing fructooligosaccharide during refrigerated storage. Int J Food Sci Technol 39: 613-621.