芥藍白化幼苗對乙烯之三相反應之研究

Studies on the Ethylene-Induced Triple Response in Etiolated Seedlings of Chinese Kale

劉先捷(Hsien-Chieh Liu)

指導教授 ： 張祖亮

共同指導教授 ： 王自存(Tsu-Tsuen Wang)

國立臺灣大學/生物資源暨農學院/園藝學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.02509

摘要

本試驗以蕓苔屬中的芥藍菜為研究材料，首先研究建立一個可以大量進行乙烯三相反應的系統，並進一步研究其對乙烯所表現出的三相反應的特性，最後以乙烯作用抑制劑1-MCP處理幼苗，証明抑制乙烯作用可以表現出類似對乙烯不敏感變種的生長狀態。
各品種幼苗三相反應對乙烯濃度與時間的表面反應分析圖顯示，外施10 ppm乙烯處理之芥藍幼苗在播種六天後表現出的三相反應與對照組有巨大的差異。
播種後第六天根長與胚軸長對於乙烯濃度的三次多項式回歸分析顯示，五個品種的芥藍可區分為兩群。第一群為白花芥藍與竹北早生芥藍，第二群則有蕙津芥藍與高腳黑芥藍，而矮腳黑芥藍則介於兩群之間。
1 ppm 1-MCP與10 ppm 乙烯組合處理下的白花芥藍幼苗在播種後第六天呈現出比對照組更沒有乙烯誘發之三相反應的結果：更小的根長、更大的胚軸長與更小的芽鉤角度。
白花芥藍與蕙津芥藍白化幼苗三相反應之內在相關性分析顯示了兩種相關的模式，白花芥藍的根長與胚軸長、胚軸長與芽鉤角度、根長與芽鉤角度呈現「正、負、無」相關的現象，蕙津芥藍的根長與胚軸長、胚軸長與芽鉤角度、根長與芽鉤角度則呈現「負、負、正」相關的現象。
將各品種芥藍無菌播種於pH 5.7的0.7 % agar培養基上，並於25

關鍵字

乙烯 ； 白化幼苗 ； 芥藍 ； 三相反應

並列摘要

The purposes of this research were firstly to set up a massive ethylene-induced triple response system of Chinese kale, secondly to study the behavior of Chinese kale in ethylene-induced triple response, and finally to apply ethylene antagonist 1-MCP on Chinese kale seedlings in order to simulate growth pattern of ethylene insensitive mutants.
The surface response analysis of triple response over ethylene and time shows that on the 6th day after planting, the triple response of 10 ppm ethylene treated Chinese kale seedlings is very significant.
The three multinomial regression analysis of the root length and hypocotyls length of 6-days-after-planting seedlings over ethylene concentration shows that, the 5 cultivars of Chinese kale can be classified to 2 groups : group 1 includes ‘White Flower’ and ‘Jubay rathe’, and group 2 includes ‘Veg Gin’ and ‘Tall Blackie’ while ‘Short Blackie can
be considered to be in either group 1 or group 2.
The 6-days-after-planting Chinese kale ‘White Flower’ seedlings under the combination of 1 ppm 1-MCP and 10 ppm ethylene treatment show no triple response with shorter roots, longer hypocotyls and smaller
hooks than the check.
The correlation analysis between triple responses on Chinese kale ’White Flower’ and Chinese kale ‘Veg Gin’ shows 2 different
correlation types.
After all, the simplest and the most reliable system to screen ethylene insensitive mutants by means of ethylene-induced triple response is to plant Chinese kale seeds on 0.7% agar (pH 5.7) , apply 10 ppm ethylene on them in a 25

並列關鍵字

etiolated Seedlings ； Chinese kale ； triple response ； ethylene

參考文獻

Abe, K. and A. E. Watada. 1991. Ethylene absorbent to maintain quality of lightly processed fruits and vegetables. J. Food Sci. 56: 1589-1592.

Aharoni, N. and M. Lieberman. 1979a. Ethylene as a regulator of senescence in tobacco leaf discs. Plant Physiol. 64: 801-804.

Aharoni, N. and M. Lieberman. 1979b. Patterns of ethylene production in senescing leaves. Plant Physiol. 64: 796-800.

Bleecker, A.B., M. A. Estelle, C. Sommerville, and H. Kende. 1988. Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana. Science 241,1086-1089.

Chang, C., S. F. Kwok, A. B. Bleecker, and E. M. Meyerowitz. 1993. Arabidopsis ethylene-response gene ETR1:similarity of product to two-component regulators. Science. 262:539-544.