Paenibacillus macerans TKU029生產生物界面活性劑之條件與特性分析

Production and characterization of biosurfactants from Paenibacillus macerans TKU029

吳佳真(Chia-Chen Wu)

指導教授 ： 王三郎

淡江大學/理學院/化學學系碩士班/碩士(2011年)

https://doi.org/10.6846/TKU.2011.00744

摘要

本研究篩選自淡水紅樹林生物界面活性劑生產菌TKU029，經由16SrDNA序列比對及API試驗鑑定為Paenibacillus macerans 。
P. macerans TKU029 生產生物界面活性劑之較適培養條件為，將含有2%烏賊軟骨粉、0.1% K2HPO4 及0.05% MgSO4．7H2O之100mL液態培養基(pH 7.21)充填於250 mL之錐形瓶，經滅菌20分鐘後進行接菌，並於30℃搖瓶(150rpm)培養3天。醱酵所得離心上清液，經鹼沈澱、甲醇萃取等步驟，進行生物界面活性劑之純化，其產量為1.76 g/L。
P. macerans TKU029所生產生物界面活性劑能將水的表面張力從72降至36.34 mN/m，其乳化活性為52∼56%且具熱穩定性。TKU029生物界面活性劑之pH安定性為4∼10、鹽度安定性為1%∼5%。微生物抑制測試顯示，TKU029生物界面活性劑對Aspergillus fumigatus、Fusarium oxysporum、E. coli、Bacillus subtilis TKU007、Staphylococcus aureus和Pseudomonas aeruginosa K187皆有抑制效果，且可應用在草莓之保存。

關鍵字

Paenibacillus macerans ； Biosurfactant ； Surface tension

並列摘要

The biosurfactant producing strain, TKU029, was isolated from Taiwanese soil and identified as Paenibacillus macerans by 16S rDNA gene sequencing and API (Analyitcal Profile Index) test.
The optimized culture condition for biosurfactant production was composed of 2% squid pen powder (SPP), 0.1% K2HPO4, 0.05% MgSO4．7H2O (pH 7.21), with autoclave treatment for 20 min, afterward, P. macerans TKU029 was incubated in 100mL of above liquid medium in a flask (250 mL) and kept shaking at 30℃ for three days. The biosurfactant was extracted from the culture supernament by alkaline precipitation, extraction of methanol. The yields of biosurfactant was 1.76 g/L.
P. macerans TKU029 biosurfactant could reduce surface tension of water from 72 to 36.34 mN/m. The emulsification index was 52∼56%. And the biosurfactant had good thermal staility and was resistant to higher temperatures. The pH stability was 4∼10, and the salinity stability was

1%∼5%. The P. macerans TKU029 biosurfactant showed antimicrobial activity against A. fumigatus、F. oxysporum、E. coli、B. subtilis TKU007、S. aureus and P. aeruginosa K187. Besides, P. macerans TKU029 biosurfactant could preserve strawberry.

並列關鍵字

Paenibacillus macerans ； Biosurfactant ； Surface tension

參考文獻

Ahimou F, Jacques P, and Deleu M (2000) Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enzyme and Microbial Technology, 27:749-754.

Amezcua-Vega C, Poggi-Varaldo H M, Esparza-Garcia F, Rios-Leal E, and Rodriguez-Vazquez R (2007) Effect of culture conditions on fatty acids composition of a biosurfactant produced by Candida ingens and changes of surface tension of culture media. Bioresource Technology, 98:237-240.

Abdel-Mawgoud A M, Mohammad M A, and Haleem H N (2008) Characterization of surfactin produced by Bacillus subtilis isolates BS5. Applied Biochemistry and Biotechnology, 150:289-303.

Arutchelvi J, Bhaduri S, Uppara P V, and Doble M (2009) Production and characterization of biosurfactant from Bacillus subtilis YB7. Journal of Applied Sciences, 9:3151-3155.

Banat I M (1995a) Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review. Bioresource Technology, 51:1-12.