脂肪酸對聚己內酯和氫氧基磷灰石支架之成孔性及生物礦化性質之研究

組織工程支架提供細胞貼附、生長及分化的立體環境，本研究以具生物可降性的聚己內酯(PCL)作為支架構成材料，為提高支架表面的親水性及骨傳導性而添加氫氧基磷灰石(HA)，並以抽真空-冷凍乾燥法形成多孔支架，但複合多孔材料內部產生相分離而降低支架的機械性質。因此，本研究以油酸(C18)作為界面活性劑，以改善複合材料相分離的程度。其中PCL支架屬於非對稱孔洞結構，內部孔洞呈45度偏斜分布，PCL-C18支架孔洞分布沒有規律，PCL-HA支架屬於對稱孔洞結構，內部孔洞呈水平分布，PCL-C18-HA支架其孔洞分佈與大小類似於PCL-C18與PCL-HA支架。測量支架的液體通透性，改變流體流動路線，可發現PCL-C18-HA支架通透性遠高於其他種類支架，推測與其孔洞結構有關。動物實驗中觀察到PCL-C18-HA支架有較薄的纖維組織產生，推測油酸與氫氧基磷灰石粒子能形成穩定產物可均勻分布在支架內部，因而減少釋放的速度，而降低組織反應的程度。並且可導引組織進入支架內部，期望未來可用於牙周及齒槽骨組織導引再生等應用。

關鍵字

氫氧基磷灰石；聚己內酯；真空-冷凍乾燥法；界面活性劑；相轉移

並列摘要

Tissue engineering scaffolds provide an three-dimensional environment for cell adhesion, growth and differentiation. In our study, a biodegradable polycaprolactone (PCL) as scaffold material and hydroxyapatite (HA) was added to improve the osteoconduction and hydrophilicity of scaffold. Porous scaffolds was prepared by vacuum - freeze drying method. Phase separations often generated in scaffold and reduce the mechanical properties. Therefore, phase separation degree of composite scaffold was decreased by oleic acid (C18) as a surfactant. The internal pore distribution of PCL scaffold is asymmetric and 45 degrees skewed. The internal pore distribution of PCL-C18 scaffold is random. The internal pore distribution of PCL-HA is symmetric and horizontal array. The internal pore distribution and pore size of PCL-C18-HA scaffold is similar to the PCL-C18 and PCL-HA. Measurement of liquid permeability of the scaffold, route changing of fluid flow, p PCL-C18-HA scaffold is much higher than other types of scaffold, lead to pore structure and distribution. An animal experiment was observed that the PCL-C18-HA scaffolds have thin fibrous tissues. Oleic acid and hydroxyapatite particles can form a stable product can be evenly distributed within the scaffold. Thus reducing the release rate of irritating substances then reduced the extent of tissue reaction and guided tissue into the scaffolds. In the future, our study can be used for the application of guided regeneration of periodontal and alveolar bone.

並列關鍵字

hydroxyapatite ； polycaprolactone ； nanocomposite ； surfactant ； colloidal stability

參考文獻

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脂肪酸對聚己內酯和氫氧基磷灰石支架之成孔性及生物礦化性質之研究

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