探討荖葉對於熱氧化沙拉油�高膽固醇飲食下大鼠血脂、肝功能與腸道菌群的影響

熱氧化的油、尤其是反覆使用的油炸油，所產生的醇類、醛類、酮類、酸類或碳氫化合物等極性化合物，與一些疾病是有相關性的，包括高血壓、脂肪肝、心血管疾病等。而先前對於荖葉的研究當中，已經證實了具有抑癌及抗突變、抗氧化、保護肝臟、降血脂的效用。而長期高油脂的飲食除了會增加身體和肝臟的重量，也極易造成脂肪肝，初期的脂肪肝病變並不會影響到肝功能指數的變化，但長期累積下來則會變成非酒精性脂肪性肝炎，非酒精性脂肪性肝炎是一個漸進的肝病，會導致肝細胞纖維化，最終進展為肝硬化。本研究的目的是探討荖葉對於使用油炸過的沙拉油餵食大鼠後對其血脂、肝功能以及腸道菌群的影響。將Sprague- Dawley雄性大鼠隨機分為三組，分別餵食以下飼料，為期十週：高油脂油炸沙拉油組(FSO)、高油脂油炸低劑量荖葉組(FSOL, FSO + Piper betle [50 mg/kg])、高油脂油炸高劑量荖葉組(FSOH, FSO + Piper betle [100 mg/kg])。十週後，犧牲大鼠，採集血液作血液生化值分析，並將其盲腸內容物進行細菌培養。實驗結果顯示荖葉具有降低血中ALT、TC指數，增加雙歧桿菌及乳酸桿菌的菌量，產生競爭性排除效應，進而改變腸道菌群生態。關鍵詞：荖葉、高油脂飲食、油炸油、雙歧桿菌、乳酸桿菌

關鍵字

荖葉；高油脂飲食；油炸油；雙歧桿菌；乳酸桿菌

並列摘要

Thermal oxidative oil, especially repeatedly used frying oil, contains free fatty acids, small molecular alcohol, aldehyde, ketone, acid, lactone and hydrocarbon, diglyceride and monoglyceride, cyclic and epoxy compounds, trans isomers those are resulted from the chemical reactions of hydrolysis, oxidation, isomerization and polymerization during deep frying. Thermally oxidized oil generates reactive oxygen species that have been implicated in several pathological processes including hypertension, fatty liver, cardiovascular disease. Piper betel has been confirmed to have tumor suppressive, anti-mutagenic, antioxidant, hypolipidemic, and hepatoprotective effects. A long-term high-fat diet no only increases body and liver weight, and also leads to fatty liver. Fatty liver disease does not affect the liver function indices at first, but cumulative effects will lead to non-alcoholic steatohepatitis, a steatosis associated with inflammation, cell death, and fibrosis which can progress to cirrhosis. The aim of present study is to investigate the effects of Piper betel on serum lipids, liver function and gut microbiota in rats fed with thermally oxidized soybean oil/ high-cholesterol diets. Male Sprague-Dawley rats were divided into three groups and were fed with the following diets, respectively, for 10 weeks: soybean oil repeatedly used in frying food (FSO), FSO diet + Piper betle [50 mg/kg] (FSOL), and FSO diet + Piper betle [100 mg/kg] (FSOH). After 10 weeks, the rats were sacrificed, and blood samples were collected for blood biochemical parameters analysis and cecal contents for bacterial culture. Experimental results show that Piper betle reduced blood ALT, and TC data, increased the amount of Bifidobacterium and Lactobacillus, produced competitive exclusion effect, thereby changing the intestinal flora ecology. Keywords：Piper betle、High-fat diet、thermally oxidized oil、Bifidobacterium、Lactobacilli

並列關鍵字

Piper betle ； High-fat diet ； thermally oxidized oil ； Bifidobacterium ； Lactobacilli

參考文獻

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探討荖葉對於熱氧化沙拉油�高膽固醇飲食下大鼠血脂、肝功能與腸道菌群的影響

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