光交聯幾丁聚醣/明膠再生膜之特性研究

Characterization of photocrosslinkable chitosan/gelatin for regeneration membrane

余奕璇(Yi-Hsuan Yu)

指導教授 ： 丁信智

中山醫學大學/口腔醫學院/口腔科學研究所/碩士(2019年)

https://doi.org/10.6834/CSMU201900108

摘要

為引導牙周及齒槽骨組織重建，使用再生膜覆蓋在骨缺損區域，避免軟組織長入骨缺損區域，如此可使骨細胞有足夠的成長空間生成新骨，以達成骨再生的目的。由於幾丁聚醣與明膠具有成膜性和生物相容性，使其廣泛應用於醫療器材。然在體溫下注射操作與固態成型，本研究使用甲基丙烯酸酐修飾幾丁聚醣再添加明膠，並通過光聚合固化。研究利用螢光胺和核磁共振來確定甲基丙烯酸化的程度，XRD與FTIR分析結構的轉變，流變分析凝膠化溫度及拉伸強度測試機械性質，此外，膨潤分析、降解測試與細胞毒性及抗菌性測試抑是分析重點。實驗結果顯示MA成功的接枝至幾丁聚醣，甲基丙烯酸化的程度約有20%的胺基被取代掉，且透過二者不同比例的調整，凝膠溫度落在33至42℃內。當複合膜中之甲基丙烯醯胺幾丁聚醣含量增加時，抗拉強度從12 MPa增加至17 MPa。各複合膜經過膨潤分析在24小時後膨潤值達75%左右，而體外降解測試結果發現複合膜在28天內降解85%左右。在實驗過程中還發現當酚類的添加不僅可提高抗拉強度，且有效降低細胞毒性但不影響抗菌能力。本研究成功透過甲基丙烯酸酐修飾幾丁聚醣，合成可光交聯的熱敏感性的複合再生膜。

關鍵字

再生膜 ； 屏障膜 ； 光交聯 ； 幾丁聚醣 ； 明膠

並列摘要

In order to guide the reconstruction of periodontal and alveolar bone tissue, the regeneration membrane is used to cover the bone defect area to avoid the soft tissue growing into the bone defect area, so that the bone cells have enough growth space to be produced new bone, so as to achieve the purpose of bone regeneration. Because chitosan and gelatin have film-forming properties and biocompatibility, they are widely used in medical materials. However, injection operating and solid state forming at body temperature, this study used methacrylic anhydride to modification chitosan and add gelatin than cured by photopolymerization. The study used fluorescamine and nuclear magnetic resonance to determine the degree of methacrylation, XRD and FTIR analysis of structural transformation, rheological analysis gelation temperature and tensile strength test mechanical properties. In addition, swelling analysis, degradation testing, cytotoxicity and antibacterial testing are the focus of analysis. The experimental results show that MA is successfully grafted to chitosan, and the degree of methacrylation is about 20% of the amino groups are replaced. And the gelation temperature falls on 33 to 42℃ by adjusting the ratio of between the two. When the content of methacrylamide chitosan in the composite membrane is increased, the tensile strength is increased from 12 MPa to 17 MPa. After swelling, the composite membranes showed a swelling value of about 75% after 24 hours, and the in vitro degradation test showed that the composite membrane degraded by about 85% in 28 days. It was also found during the experiment that the addition of phenols not only improved the tensile strength, and effectively reduce cytotoxicity, but does not affect the antibacterial ability. In this study, the chitosan was modified by methacrylic anhydride to synthesize a photocrosslinkable thermosensitive composite regeneration membrane.

並列關鍵字

Regeneration membrane ； Barrier membrane ； Photocrosslinking ； Chitosan ； Gelatin

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