聚麩胺酸/明膠之交聯複合水膠研究

皮膚傷口在適量潤濕狀態下的癒合速度比其處在乾燥狀態要來的快，但在癒合過程中，若有過多的自由基則會導致發炎反應過度發生，而造成傷口癒合速度減慢，因此想要將具有凝膠特性的明膠，結合有著高吸水及保濕性的聚麩胺酸鹽，並利用具有強抗氧化性的原花青素進行交聯反應，製成天然高分子共聚物水膠，使之具有高吸水、保濕、抗氧化性，並應用於創傷敷料。本實驗製備含γ-聚麩胺酸鈉/鈣鹽、明膠之水膠敷材，以原花青素交聯，進行細胞實驗與動物實驗以評估其生物相容性。實驗結果顯示，經原花青素交聯後的水膠，不僅機性結構有所提升，抗氧化能力也大幅增加。體外生物適應性結果顯示，添加10 wt%原花青素的水膠對L929纖維母細胞不具有毒性。最後由體內生物測試等實驗結果得知，本研究所發展出之水膠敷材能增加傷口復原速率，顯示經原花青素交聯的γ-聚麩胺酸鈉/鈣鹽、明膠之水膠為一具潛力的傷口癒合敷材。

關鍵字

敷料；明膠；低聚合原花青素；聚麩胺酸鹽；細胞實驗；動物實驗

並列摘要

The healing of wounds on skin is accelerated under a proper humid environment than a dry one. Nevertheless, immoderate free radicals will lead to excessive inflammation reactions and retard the healing processes. The motivation of this study is to prepare a cross-linked composite hydrogel with combining the advantages of gelation gelatin, humidifying γ-poly glutamate (γ-PGA) and antioxidative proanthocyanidins (OPCs). The natural polymer made hydrogel is proposed to be applied as a wound dressing. In this research, the hydrogel wound dressing was prepared by using sodium/calcium γ-PGA and gelatin cross-linked with OPCs. The optimized ratio of γ-PGA salt to gelatin was evaluated through swelling and degradation tests. Furthermore, the hydrogel was cross-linked with OPCs and then tested in vitro and in vivo. Results indicated that the cross-linked hydrogel using OPCs solution had stronger machine structure and improved antioxidant capacity significantly. The results of in vitro tests showed that the cross-linked hydrogel using 10% OPCs solution would be no bio-toxic for L929 cell. The results of in vivo tests showed that the cross-linked hydrogel using 10% OPCs solution would be of great potential to serve as a useful wound healing dressings.

並列關鍵字

Hydrogel Wound Dressing ； γ-PGA ； Gelatin ； In Vitro Study ； In Vivo Study

參考文獻

50. 藥品非臨床試驗安全性規範，行政院衛生署，2000。

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3. Gurtner, G.C., Werner, S., Barrandon, Y., & Longaker, M. T., Wound repair and regeneration. Nature, 2008. 453(7193): p. 314-321.

4. Halliwell B, G.J., ed. Free radical in biology and medicine. . 3rd ed. 1999, Clarendon press, oxford.: New York.

5. Hinman CD, M.H., Effect of air exposure and occlusion on experimental human skin wounds. Nature, 1963. 200: p. 377-378.

國際替代計量

聚麩胺酸/明膠之交聯複合水膠研究

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