- 學位論文
桑葉水萃抑制非酒精性脂肪肝以及酒精性脂肪肝
The Extracts of Mulberry Leaves inhibits Non-Alcoholic Fatty Liver Disease and Alcoholic Fatty Liver Disease
摘要
脂肪性肝病正嚴重威脅國人的健康，相關問題也日漸引起國人的注意。而現代人的飲食習慣正是造成脂肪肝的元凶，脂肪肝之所以必須重視正是因為過多的脂肪堆積在肝臟會影響肝臟的功能，也容易造成肝病變的發生。桑葉過去已被證實具有抗氧化、降低血脂等功效，但是桑葉水萃(MLE)對於降低脂肪肝生成之功效尚未有研究做深入探討。因此本實驗將針對MLE減緩脂肪肝生成之作用，分成非酒精性脂肪肝與酒精性脂肪肝兩方面去評估。首先利用高脂飲食配合MLE餵食Wistar大鼠之模式評估MLE對於脂肪肝生成的影響；另外再以Lieber-DeCarli 酒精飼料配合MLE餵食C57BL/6J 小鼠的方式評估MLE抑制酒精性脂肪肝之作用。實驗期間紀錄實驗動物之體重變化、血清AST、ALT、TG、Cho、周邊脂肪含量、體脂肪、肝臟抗氧化酵素和肝臟脂質過氧化等。結果發現，在兩種模式中給予MLE確實均達到減緩脂肪肝生成的情形，而MLE還可以調節酒精脫氫酶(ALD)和乙醛脫氫酶(ALDH)的活性變化，尤其在餵食高劑量2% MLE的組別中更為顯著。總和以上結果，MLE確實具有預防非酒精性脂肪肝以及酒精性脂肪肝生成之作用，未來將可進一步發展成保護肝臟的保健食品。
並列摘要
Non-alcoholic fatty liver disease (NAFLD), a leading cause of liver damage, comprises of a spectrum of liver abnormalities including the early fat deposition in the liver (hepatic steatosis) and advanced nonalcoholic steatohepatitis (NASH). In addition, alcoholic liver disease (ALD) also lead to similar liver syndrome with NASH.In previous studies, mulberry leaves had been demonstrated to possess anti-oxidantant promoting effects. In this study, we have examined the effect of water extracts of mulberry leaves (MLE) on HFD-induced hepatic steatosis in rats. We used two animal models to do our research: NASH and ALD models. The rats were fed either a rodent normal chow, chow containing high-fat diet (HFD), or HFD containing 0.5% or 1% and 2% MLE in the diet for 10 weeks. Moreover, we first fed the HFD diet for 4 weeks to induce hepatic steatosis, then treated with MLE (0.5% or 1% and 2% in the diet) in the HFD diet for 6 weeks. In ALD model, we fed Lieber-DeCarli alcohol liquid feed for 8 weeks. We assayed antioxidant enzyme activity, such as SOD, catalase, GSH peroxidase. Furthermore, we also used the ELISA kits to measure the inflammatory factors, such as IL-6, TNF-α and the fat-relative factors, as adiponectin, leptin. Our data indicated that both NASH and ALD model are inclusion of 0.5%, 1% and 2% MLE in the HFD diet decreased liver fat content, liver weight, hepatic oxidative products, and prevented hepatic steatosis significantly. In serum, the AST, ALT and total triglyceride, total cholesterol decreased significantly in treating MLE group. MLE increased anti-oxidant enzyme activities in liver of HFD-induced rats. We also found that inflammatory factors TNF-d and the fat-relative factors, such as adiponectin, leptin, reduced in the treating MLE groups. These findings suggested that MLE effectively prevented and caused the regression of experimental hepatic steatosis. In the future, we could find the way to do more research about finding more effective compounds from mulberry leaves so that we would have more solution about treating liver disease.