基質金屬蛋白酶11其基因多型性與口腔癌相關性探討

癌症為台灣地區十大死亡原因之冠，在台灣，口腔癌在十大癌症死因中排名第五名，特別在男性中口腔癌死亡率更是排名第四位，由此可見口腔癌在台灣地區造成的高死亡率，近年來更有逐年上升的趨勢。在過去的研究中發現，基質金屬蛋白酶11(Matrix metalloproteinase 11；MMP-11) 可以增加腫瘤所引起的血管新生，或是藉由調控細胞的增殖、轉移、侵襲、凋亡，對腫瘤的生長與癌症的發展有密切的相關。經由臨床檢體的證明，在各個器官的原發性腫瘤中，MMP-11大量表現的患者在治療後會有較差存活率及預後。所以，本研究中我們選取了595位口腔癌患者以及561位健康對照組，蒐集年齡、性別、嚼食檳榔、飲酒以及抽菸情形的資料後，萃取其血液中的DNA檢體利用即時定量聚合酶連鎖反應(Real-time PCR)進行對於MMP-11基因rs738791、rs2267029、rs738792以及rs28382575的基因多型性進行分析，同時更進一步納入口腔癌病人其臨床血清指標以及癌症分期，嘗試分析以上基因其多型性與口腔癌發展之相關性。結果發現，在臨床參數的分析中，MMP-11基因rs738792的基因多型性攜帶CC/CT基因型相較於TT基因型的患者對腫瘤在淋巴結轉移可能存在較高的危險。然而，MMP-11基因多型性如何影響口腔癌患者淋巴結轉移的機制仍尚未明瞭，需要更多對於基因多型性的研究同時結合對機制的探討來進一步釐清MMP-11基因多型性在口腔癌中所扮演的角色。相信若能釐清這兩者之間的相關及其中詳細的機制，對於口腔癌的防治以及治療上必定有很大的幫助。

關鍵字

基質金屬蛋白酶11 ；口腔癌；基因多型性

並列摘要

Oral squamous cell carcinoma (OSCC), a common malignant cancer, is the 4th common cancer in males and the 5th leading cause of cancer death in Taiwan. According to the official statistics, the death rate caused by OSCC has been increased in recent years. In previous studies, matrix metalloproteinase 11 (MMP-11) has been shown to increase the angiogenesis and regulate cancer cells proliferation, metastasis, invasion, and apoptosis. In addition to those studies, some clinical information indentified the high MMP-11 expression level in original tumors cause poor survival rate and prognosis. In this study, we recruited 595 OSCC patients as the cased group and 561 non-cancer individuals as the control group and collected the information about age, sex, betel-quid chewing, alcohol consumption and tobacco use. To analysis allelic discrimination of the rs738791, rs2267029, rs738792 and rs22382575 polymorphisms of the MMP-11 gene, we used DNA samples from both groups and analyzed by real-time polymerase chain reaction. As the results, we found that the rs738792 polymorphism of MMP-11 gene in OSCC patients might increase the risk of lymph node metastasis. However, the reliable mechanism regulates the lymph node metastasis of OSCC by MMP-11 gene polymorphisms still unclear. To understand the relationship between MMP-11 polymorphisms and metastasis of OSCC and the underlying mechanism may provide a helpful prevention and medical therapy for OSCC.

並列關鍵字

MMP11 ； oral cancer ； SNP

參考文獻

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基質金屬蛋白酶11其基因多型性與口腔癌相關性探討

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