明膠(gelatin)是一種膠原蛋白的衍生物,其組成及性質與膠原蛋白相似,因此擁有極好的生物相容性以及生物可降解性。碳酸鈣(CaCO3)及磷酸鈣(CaHPO4)擁有良好的生物相容性,且為人體骨骼中的主要成分,近年來兩者都廣泛使用在骨修補材料之中。本實驗目的在於改進現行的乳牙根管充填材料(Vitapex),加強材料的生物相容性以及生物可降解性,降低對人體造成的發炎反應,並且提高細胞的助生性。本實驗利用細胞相容性測試、細胞週期分析以及西方墨點分析等方法,分析三種添加明膠的鈣基化合物對於細胞活性與細胞發炎反應的差異性表現,並且與Vitapex比較分析。結果發現,碳酸鈣以及磷酸鈣添加明膠的混和材料對於細胞的生物相容性在統計學上與Vitapex有顯著的差異,又以磷酸鈣添加明膠的生物相容性為最佳。Vitapex與氫氧化鈣一開始對於細胞的刺激性較高,而磷酸鈣添加明膠的混和材料在細胞週期分析的實驗中,對於細胞週期速度有正面的影響性。Vitapex以及氫氧化鈣一開始對於發炎細胞的發炎程度會增加,而磷酸鈣添加明膠的混和材料在第24小時的發炎程度為最小。磷酸鈣加上明膠的混和材料為本實驗中效果最佳者,未來可以運用在動物實驗中,進而開發出更適合人類的骨科修補材料。
Gelatin is derived from collagen, and it’s component and property are similar to collagen. Therefore gelatin has great biocompatibility and biodegradable. CaCO3 and CaHPO4 both have outstanding biocompatibility, and are main component of bone. Recently, CaCO3 and CaHPO4 are used to bone repairing material popularly. The purpose of this study is to improve vitapex which is a kind of milk teeth root canal filling material, enhancing biocompatibility and biodegradable of material, reducing inflammation reaction and then increase cell proliferation. This study use cell compatibility test、cell cycle test and western blot to analysis three calcium based materials with gelatin which make response to cell vitality and cell inflammation reaction. Furthermore, they will compare with vitapex. The result of cell compatibility test reveal there is significant difference between CaCO3 、CaHPO4 with gelatin and vitapex(p<0.05), especially for CaHPO4 with gelatin. Vitapex and Ca(OH)2 have more cell irritation in the initial stage. In the cell cycle anaylysis CaHPO4 with gelatin has positive effect regarding cell cycle rate. Vitapex and Ca(OH)2 increase degree of the cell inflammation early days, and CaHPO4 with gelatin cause the minimal degree of cell inflammation at first 24 hours.