以人體試食試驗評估益生菌果蔬纖維粉在腸胃道保健及免疫調節之研究

現今人因生活壓力大、活動量的降低，容易造成免疫力下降引發感冒症狀，且因飲食習慣不佳產生許多腸胃道相關問題，如:胃潰瘍、便秘、腹瀉等。先前研究證實益生菌能穩定腸道屏障、增加周邊血液淋巴球活性進而免疫力及改善腸胃道問題等功能。近年來人們健康意識抬頭，提倡補充益生菌來提升免疫力及改善胃腸道之功能，若要使良好的菌群定存於腸道，則需要幫助其生長之物質-益生元的攝取。因此，本研究將以益生菌、益生元、果蔬纖維粉組合配方，探討其對腸胃道保健與提升免疫力之作用。本研究為一項隨機、單盲、安慰劑對照組平行試驗，招募50 位過去一年至少感冒4 次或曾經有胃幽門螺旋桿菌感染、胃潰瘍之男女性，隨機分派至兩組 (n=25/組)，分別為 (1) 安慰組 (Placebo)、(2) 益生菌果蔬纖維粉組 (Probiotics fruit vegetable fiber powder, PF, 4.48×1011 CFU/person/day)，實驗為期12 週。實驗結果發現介入前兩組之間的血液基本生化數值、體位測量、飲食分析皆尚未出現差異，而免疫相關參數分析，PF 組在介入12 週後相較於未食用前可顯著增加IgG、IgM 以及IL-10，另外可顯著降低IL-6、IL-12、IFN-γ及TNF-α，具有調節免疫力之功效。體感問卷部分，PF 介入12 週後相較於食用前可顯著增加腸胃道蠕動頻率、改善脹氣、胃痛、糞便堅硬、便秘以及腹瀉之問題。另外，可減少頭痛、肌肉疼痛及頭暈之狀況，以及改善皮膚敏感之趨勢。此外，還可降低與發炎相關反應呈現正相關之菌株，並增加抗發炎之菌株。由上述結果證實，益生菌果蔬纖維粉組在介入前後之血液免疫數值、腸胃道問題及腸道菌相有顯著改善，具有腸胃道保健及提升免疫力之功效。

關鍵字

益生菌；果蔬纖維；免疫調節；腸胃道保健

並列摘要

Nowadays, people are liable to catch cold due to high pressure and less physical activity resulting in immunity deficiency. Consequently many gastrointestinal problems due to unbalance food intake, would occur such as gastric ulcer, constipation, diarrhea, etc. Previous studies have confirmed that probiotics can stabilize intestinal barrier, increase the activity of peripheral blood lymphocytes, and improve gastrointestinal functions. In recent years, promoting the probiotics supplement can enhance immunity and improve the intestinal function. Therefore, this study applied the combination formula of probiotics, prebiotics, and fruit vegetable fiber powder (PF), which aimed at the investigation of the effects of PF on gastrointestinal function and immune regulation. The randomized, single-blind and placebo-controlled study conducted on 50 adults in 12 weeks. They were randomized allocated to either placebo or PF group. The inclusive criteria were male or female participants to be aged at 20 to 60 years old, who had colds more than four times in the past year or had gastric infection by helicobacter pylori or peptic ulcer. The participants received such as containing either PF (4.48×1011 CFU/person/day) or placebo (maltodextrin) two times a day for 12 weeks. The results showed that the blood biochemical parameters, body position measurements, and dietary intake between two groups before intervention had no statistical difference (p>0.05). Compared to week 0, PF group had significantly increase serum IgG, IgM, and IL-10 at week 12 (p<0.05). Moreover, it can significantly decrease IL-6, IL-12, IFN-γ, and TNF-α after intervention for 12 weeks (p<0.05). In other words, the PF showed the effect to regulate immunity. In addition, after 12 weeks of intervention on PF group can tell the frequency of gastrointestinal motility had significantly increased, and also improve flatulence, gastralgia, hard stools, constipation, and diarrhea compared to week 0 (p<0.05). Furthermore, PF can reduce headache, muscle pain, and dizziness (p<0.05). In microbiota, bacterial strains of inflammation related bacteria were reduced, and increased anti-inflammatory strains. In conclusion, the PF group significantly improved blood immune paramaters, gastrointestinal problems and fecal microbiotia after 12 weeks intervention, which has the effect of gastrointestinal health and immunity regulation.

並列關鍵字

probiotics ； fiber ； immune regulation ； gastrointestinal health

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