兔兒菜(Ixeris chinensis (Thunb.) Nakai),是民間普遍使用的 草藥材。本研究擬探討兔兒菜各種萃取物之抗氧化能力以及抑制幽門 螺旋桿菌附著和入侵人類胃上皮細胞(gastric epithelial cells AGS cells)之能力。 結果顯示,兔兒菜熱水萃取物(boiling water extract, BWE)在抗 氧化實驗中表現具有較佳的抗氧化活性,其次為乙醇萃取物(ethanol extract, EE)及氯仿萃取物(chloroform extract, CE)。熱水萃取物含有最多量之總酚及最高量之總類黃酮。而酚類化合物的高效能液相層析(high-performance liquid chromatogram, HPLC)結果顯示,熱水萃取物的酚類化合物含量遠高於其他兩者。銅離子誘導人類低密度脂蛋白(low-density lipoproteins, LDL)的氧化作用中,熱水萃取物及乙醇萃取物展現極佳的抑制作用。然而,氯仿萃取物具有較佳的幽門螺旋桿菌抑菌能力。在對抗幽門螺旋桿菌誘導胃上皮細胞發炎的試驗可知,熱水萃取物與氯仿萃取物具有良好的抗發炎活性。此外,熱水萃取物有助於腸道益生菌(Lactobacillus spp. 和Bifidobacterium spp. )之生長並抑制有害菌(Clostridium perfringens 和 E. coli )的效果,而乙醇萃取物則僅具有抑制有害菌之活性。
Ixeris chinensis (Thunb.) Nakai is traditional herb medicine. The goal of the study is to evaluate the antioxidant activity and inhibition on the adhesion/invasion of Helicobacter pylori (H. pylori) to AGS cells. Our study demonstrated that boiling water extracts(BWE) showed better antioxidion, than those of ethanol extracts(EE) and chloroform extracts(CE). BWE also contained high level of phenolics and flavonoids. The lag time of human low-density lipoprotein (LDL) oxidation induced by copper who greatly increased by BWE, EE and CE. CE showed inhibition and bactericidal effect on H. pylori. However, the effect on H. pylori-induced gastric epithelial cells inflammation was found by BWE and CE. In addition, BWE could increase the numbers of probiotics (Lactobacillus spp. and Bifidobacterium spp. ), BWE and CE could inhibit the growth of harmful bactericidal (Clostridium perfringens and E. coli ).