Screen-printed carbon electrodes (SPCE) play an important role in academic research and development of commercial sensors because of the advantages of low cost, simple process, freedom of modeling and one-time use. However, carbon powder was attached to the electrode substrate of SPCE with the use of hydrophobic macro molecular, adhesive, affecting the reproducibility and sensitivity of SPCE. In this study, poly-L-lysine, poly-melamine, and gold nanoparticles were modified on the surface of the electrode to change the electrochemical properties of SPCE and storage stability. The feasibility study of β-agonist detection was investigated using the modified SPCE.