牙齦纖維母細胞在矯正用黏著劑作用下第二型環氧化酶之表現

Cyclooxygenase-2 expressions of orthodontic adhesives on primary human gingival fibroblasts

李涵鈺(Han-Yu Li)

指導教授 ： 高嘉澤

中山醫學大學/口腔醫學院/口腔材料科學研究所/碩士(2006年)

https://doi.org/10.6834/CSMU.2006.00091

摘要

本篇研究的目的，是藉由COX-2之表現，來評估不同的矯正用黏著劑對於人類牙齦纖維母細胞產生刺激的嚴重程度。0.1%黏著劑浸泡液作用在人體牙齦組織之初代纖維母細胞上，以RT-PCR和西方點墨法進行COX-2 mRNA與蛋白質之測量。利用單因子變異數分析(One-Way ANOVA)配合多重比較事後檢定法(Tukey)做統計處理，以p值小於0.05為達到統計上的顯著差異。實驗結果顯示，牙齦纖維母細胞在樹脂類和玻璃離子類黏著劑之聚合產物作用下，細胞的形態與數目都沒有產生變化；牙齦纖維母細胞COX-2 mRNA的表現在細胞與材料接觸6小時以內會達到最大；牙齦纖維母細胞COX-2蛋白質的表現在細胞與材料接觸24小時後最大，其中以樹脂類黏著劑的底劑和玻璃離子類黏著劑的粉劑尤其明顯。矯正用黏著劑的確會因為其組成分之毒性，而對人類牙齦纖維母細胞產生刺激，在臨床操作上，必須要注意盡可能地避免黏著劑與牙齦的接觸，並且在光照聚合時，要讓黏著劑充分地聚合完全。

關鍵字

牙齦纖維母細胞 ； 矯正用黏著劑 ； 環氧化酶

並列摘要

To be as an ideal adhesive, it is required biocompatibility and with no irritating to surrounding tissues. The purpose of present study was to determine the COX-2 mRNA and protein expressions of two orthodontic bonding adhesives (TransbondTM XT and Glass Ionomer Orthodontic Band Cement) on primary cultured human gingival fibroblasts. Two orthodontic bonding adhesives were immersed in 10 ml DMSO and their extracts were diluted to 0.1% concentrations in test. COX-2 mRNA expressions were quantified by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). COX-2 protein expressions were quantified by Western Blot analysis. The one way analysis of variance (ANOVA) was used as statistical method and with p<0.05 showed statistical difference. The expression of COX-2 mRNA in primary human gingival fibroblasts showed no statistical difference within control and two adhesives. On the contrary, the expression of COX-2 protein in primary cultured human gingival fibroblasts was varies with the tested adhesives. The expression of COX-2 protein showed time-dependent relationship. The activation of COX-2 protein expressions could be one of potential mechanisms of orthodontic bonding adhesives which could induce gingival inflammation.

並列關鍵字

Cyclooxygenase ； Orthodontic adhesives ； Gingival fibroblasts

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