- 學位論文
龍葵水萃取物對四氯化碳所誘發肝損傷大白鼠護肝功能之探討
THe hepatoprotective effects of the water extract of Solanum nigrum L.on hepatic damages induced by Carbon Tetrachloride in rats
摘要
惡性腫瘤是當今世界上威脅人類最嚴重的疾病之一，其中肝癌是男性首位癌症死因，女性的第二位癌症死因，國人罹患肝癌之比例為全球之冠，其對國人健康威脅之鉅可見一般。因此本研究針對化學性肝損傷進行龍葵護肝功能之探討。 龍葵是一種廣泛被用來治療癌症的中草藥植物，對肝癌、肺癌、胃癌及膀胱癌等均具有顯著療效，亦有報導指出，其具有保肝、抗發炎的效果，然而龍葵對於肝臟代謝毒物的影響及作用機轉並無確切可引用的報告。因此，本研究參考行政院衛生署「健康食品安全及功效評估方法」中之『健康食品之護肝功能評估方法」，以四氯化碳（CCl4）誘導大白鼠產生慢性肝損傷，同時投予不同濃度之龍葵水萃取液，探討龍葵水萃取液對慢性肝損傷大白鼠的影響。以測定AST、ALT、cholesterol、triglyceride、ALP、total-bilirubin等生化值作為肝損傷的指標，並分析血液中自由基之變化情形，再觀察肝組織之形態變化以探討龍葵水萃取液對於四氯化碳造成的肝細胞毒性是否具有抑制作用，及參與細胞外基質降解之基質金屬蛋白酵素表現，其所影響之代謝機轉。 實驗結果顯示，投予龍葵水萃取液之實驗組與四氯化碳控制組比較發現，投予龍葵水萃取液之老鼠血液中AST、ALT、cholesterol、TG、total-bilirubin、ALP、Superoxide及Hydroxyl radical均有改善效果，其體重之成長率也與正常組相似，病理組織切片結果亦顯示肝纖維化情形明顯獲得改善。因此推測龍葵對於四氯化碳造成的肝細胞毒性與損傷具有抑制作用，可有效保護肝臟。然而其機制及所影響之代謝機轉仍須深入分析與探討。
並列摘要
The effects of dietary administration of the water extract of Solanum nigrum Linne（SN） on the hepatic damage induced by carbon tetrachloride（CCl4）in rats were investigated. Male SD rats were treated with 0.1ml/100g body weight of 20% CCl4 by i.p. and the different degrees of hepatic damage were observed by the elevated levels of serum marker enzymes such as aspartate aminotransferase（AST）, alanine aminotransferase（ALT）, and other changes of serum components such as total cholesterol, triglyceride（TG）, ALP, Total-bilirubin. Treatments of the animals with SNL were 20mg /100g, 50mg /100g, and 100mg /100g body weight in the diets for six consecutive weeks, and the enzyme elevations were significantly suppressed. This suggested that SNE possessed chemopreventive effects on the chronic hepatic damage induced by CCl4 . It can also be observed from the histopathological evaluation of rat livers that hepatic damage is reversed in the manner of extract-dose-dependent. However, the free radical were significantly decreased, indicating SNE can provide the protection effects on the hepatic damage induced by CCl4. Via these studies, we demonstrate that SNL is a chemopreventing and hepatoprotective agent.
參考文獻
延伸閱讀
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