桑椹花青素萃取物在diclofenac誘導大白鼠胃黏膜損傷的預防作用

The preventive effect of Mulberry anthocyanin extracts in diclofenac-induced rat gastric mucosa damage

周欣敏(Hsin-min Chou)

指導教授 ： 李彗禎

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2009年)

摘要

Diclofenac是一種非類固醇抗發炎藥物，會有引起胃黏膜損傷的副作用。某些食物成分具有保護胃腸道的功能，我們評估桑椹花青素是否能減緩Diclofenac所引起的大鼠胃黏膜損傷。大鼠餵食Diclofenac (100 mg/kg)以誘導胃黏膜損傷，事先給予不同劑量的桑椹花青素 (100mg/kg; 200mg/kg; 400mg/kg)和預防藥物氫離子幫浦抑制劑Esomeprazole (500 ug/kg)當作保護參考。桑椹花青素的保護功能藉由分析血液中白血球數量和紅血球抗氧化酵素glutathione-S- transferase (GST total ; alpha ; pi ; mu )活性;分析胃黏膜脂質過氧化物、抗氧化物glutathione濃度和環氧酶-2 (COX-2)蛋白表現;胃部組織作組織病理檢查。以DIC+MAE實驗組和DIC藥物誘導組相比較，發現紅血球抗氧化酵素(GST total ; alpha ; pi )活性和胃黏膜抗氧化物濃度顯著增加，而白血球數量、胃黏膜脂質過氧化物濃度和環氧酶-2蛋白表現顯著減少。組織病理檢查更加確認桑椹花青素的胃腸保護功能。桑椹花青素能減緩Diclofenac所引起的大鼠胃黏膜損傷，或許是因為本身抗氧化特性和捕捉活性氧自由基的能力，所以桑椹花青素可當作胃腸保護劑。

關鍵字

桑椹花青素 ； 胃黏膜損傷

並列摘要

Diclofenac is a NSAID drug which causes gastric mucosal lesion complication. Food bioactive compounds were reported could exert beneficial effects in the gastrointestinal tract. In this study, we evaluated whether Mulberry anthocyanin extracts (MAE) reduced Diclofenac-induced injury in the rat gastric mucosa. Rats were induced for gastric mucosal lesion with Diclofenac (DIC, 100 mg/kg, orally) after pretreated with MAE in various dosages (100mg/kg, 200mg/kg, 400mg/kg). A proton pump inhibitor, Esomeprazole, was used as a protection control. The gastroprotective effect of MAE was assessed by WBC count in whole blood and the activities of enzymatic antioxidants, such as glutathione-S- transferase (GST, GST α, GST π, GST μ isoforms ) in RBC .The levels of lipid peroxide, reduced glutathione (GSH), and protein expression of cyclooxygenase-2 (COX-2) in gastric mucosa were also evaluated. The stomach tissues were used for the histological examination. The results showed that pretreatment of MAE can significantly increase the activities of total GST, GST α, GST π, and GSH, and decrease the WBC number, lipid peroxidation, and protein expression of COX-2 as compared to DICtreated rats. Histological studies showed a consistent result as well as the antioxidative molecules described above. Taken together, MAE reduced Diclofenac-induced gastric mucosal lesion maybe due to its antioxidative characteristic and free radical scavenging activity, and it possesses a potential to act as gastroprotective agent.

並列關鍵字

Mulberry anthocyanin ； diclofenac ； gastric mucosa damage

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