荷葉多酚及其主要成分quercetin-3-O-glucuronide促進小鼠海馬迴以及SH-SY5Y增生之研究

Nuciferine leaf polyphenol extracts and principal component Q3GA promotes proliferation in mice hippocampus and SH-SY5Y cell

陳信融(Sin-Rong Chen)

指導教授 ： 王朝鐘

中山醫學大學/醫學院/生化微生物免疫研究所/碩士(2018年)

摘要

實驗室過去發現荷葉多酚(NLPE) 之主要成分為Miquelianin (Q3GA)，國外學者已證實Q3GA於小鼠海馬迴具有促進神經細胞增生之能力，也能促進體外培養之神經幹細胞增生，而促進成年神經增生(adult neurogenesis)，是未來改善老化以及大腦損傷的希望。本研究之目的為評估荷葉多酚及其主要成分Q3GA促進神經細胞增生之功效，並以體外與活體內進行驗證。本實驗採用萃取之荷葉多酚進行動物實驗，荷葉多酚能夠增加C57BL/6小鼠之海馬迴增生，並增加Akt蛋白之活化。荷葉多酚也能增加Cyclin A, E蛋白、降低Cyclin D1之表現。荷葉多酚與Q3GA皆能增加SH-SY5Y之增生，而Q3GA能增加NGFR以及PI3K/Akt路徑之蛋白表現。Q3GA也能增加Cyclin E蛋白、降低Cyclin D1之表現。利用Akt1/2激酶抑制劑驗證p-Akt對於細胞增生扮演一定角色。基於以上實驗我們認為荷葉萃取物具有促進神經細胞增生之能力，而荷葉於國內易於種植。因此，本研究成果可用於發展增記憶、防失智之健康食品，並期望能改善全球高齡化所帶來之衝擊。

關鍵字

荷葉多酚 ； 海馬迴 ； 神經 ； 增生

並列摘要

In the past, the laboratory found that the main component of lotus leaf polyphenols (NLPE) is Miquelianin (Q3GA). Foreign scholars have confirmed that Q3GA has the ability to promote neuronal cell proliferation in mouse hippocampus, and also promotes neural stem cell proliferation in vitro. Adult neurogenesis is the hope of improving aging and brain damage in the future. The purpose of this study was to evaluate the efficacy of lotus leaf polyphenols and its main component, Q3GA, in promoting neuronal proliferation and to be validated in vitro and in vivo. In this experiment, the extracted lotus leaf polyphenols were used for animal experiments. The lotus leaf polyphenols increased the hippocampal gyrus proliferation of C57BL/6 mice and increased the activation of Akt protein. Lotus leaf polyphenols also increase Cyclin A, E protein and reduce the performance of Cyclin D1. Both lotus leaf polyphenols and Q3GA can increase the proliferation of SH-SY5Y, while Q3GA can increase the protein expression of NGFR and PI3K/Akt pathway. Q3GA also increases Cyclin E protein and reduces the performance of Cyclin D1. The use of Akt1/2 kinase inhibitors to verify that p-Akt plays a role in cell proliferation. Based on the above experiments, we believe that lotus leaf extract has the ability to promote nerve cell proliferation, and lotus leaves are easy to grow in Taiwan. Therefore, the results of this study can be used to develop healthy foods that increase memory and prevent dementia, and hope to improve the impact of global aging.

並列關鍵字

NLPE ； hippocampus ； nerve ； proliferation ； Q3GA ； Miquelianin

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