槲皮素複合補充劑對高脂飲食誘導肥胖大鼠之不易形成體脂肪作用及其分子機制探討

Antiobesity effects and molecular mechanisms of quercetin-rich supplement on high-fat diet-induced obese rats

周佩萱(Pei-Hsuan Chou)

指導教授 ： 徐慶琳

中山醫學大學/健康管理學院/營養學研究所/碩士(2014年)

摘要

肥胖對健康造成許多不利之影響，其已成為全世界嚴重的公共衛生問題之一。槲皮素 (quercetin)、牛磺酸 (taurine)、茄紅素 (lycopene) 和荔枝花萃取物 (litchi flower extract)，在抗肥胖之相關研究已廣泛被報導。然而以複方組合機能性成分已知可透過加乘作用，使其具有提高功效性和降低使用劑量之優勢。本研究將以四種成分組合之槲皮素複合補充劑 (quercetin-rich supplement)，探討其對於高脂飲食誘導肥胖大鼠之影響。結果顯示，槲皮素複合補充劑給予肥胖大鼠後，可顯著抑制高脂飲食所誘導之體重、肝臟重量和脂肪組織重量增加，並降低肝臟總脂質、三酸甘油酯和膽固醇含量，以及增加糞便總脂質、三酸甘油酯和膽固醇之排出。在肝臟和脂肪組織切片中，槲皮素複合補充劑可顯著抑制高脂飲食所誘導肥胖大鼠之油滴堆積和脂肪細胞肥大。此外，給予槲皮素複合補充劑能顯著降低血清丙二醛濃度，並提升血清總抗氧化能力和肝臟抗氧化酵素活性。在脂肪組織之基因表現上，槲皮素複合補充劑具有提升脂質合成抑制基因 (SIRT1) 之基因表現，進而抑制脂質生合成 (PPAR-γ、SREBP-1c、ACC、FAS和HMG-CoA reductase)、脂質運輸與代謝 (aP2、FATP1和LPL) 和脂肪細胞激素 (PAI-1和TNF-α) 之基因表現，以及提升脂肪酸氧化 (PPAR-α、ACO和CPT-1) 之基因表現。在肝臟之基因表現上，槲皮素複合補充劑具有抑制膽固醇合成 (HMG-CoA reductase) 之基因表現，並且可提升脂肪酸氧化 (AMPK、PPAR-α和ACO) 和脂質分解 (HSL和ATGL) 之基因表現。綜合以上結果證實，槲皮素複合補充劑確實具有抑制肥胖大鼠體內脂質生合成之作用，未來能進一步開發成具有抗肥胖潛力之保健食品。

關鍵字

高脂飲食 ； 大鼠 ； 槲皮素 ； 茄紅素 ； 牛磺酸 ； 荔枝花萃取物

並列摘要

Obesity has been recognized as a serious public health problem, associated with several health risks. Quercetin, lycopene, taurine, or litchi flower extract have been reported strong potential anti-obesity activity. However, functional formulas exhibits the strongest synergistic effect compared to single supplement. In the present study, the anti-obesity effects of quercetin-rich supplement, which were combined with quercetin, lycopene, taurine, and litchi flower extract, on high-fat diet (HFD)-induced obese rats were investigated. The data indicated that the weights of body, adipose tissues, and liver, and hepatic total lipid, triglyceride, and cholesterol in quercetin-rich supplement groups were significantly decreased as compared with the HFD group. Fecal total lipid, triglyceride, and cholesterol in quercetin-rich supplement groups were significantly increased as compared with the HFD group. In histological analysis of liver and adipose tissue, quercetin-rich supplement groups were significantly decreased in lipid accumulation and adipocyte size as compared with the HFD group. However, quercetin-rich supplement groups were significantly decreased serum malondialdehyde (MDA), and increased the levels of serum trolox equivalent antioxidant capacity (TEAC) and hepatic antioxidant enzymes as compared with the HFD group. In gene expressions of perirenal adipose tissue, quercetin-rich supplement groups were increased the gene expressions of SIRT1, PPAR-γ, ACO, and CPT-1, as well as decreased the gene expressions of PPAR-α, SREBP-1c, ACC, FAS, HMG-CoA reductase, aP2, FATP1, LPL, PAI-1, and TNF-α as compared with the HFD group. In gene expressions of liver, quercetin-rich supplement groups were also decreased the gene expression of HMG-CoA reductase and increased the gene expressions of AMPK, PPAR-α, ACO, ATGL, and HSL as compared with the HFD group. These results demonstrate that intake of quercetin-rich supplement can be developed as a potential nutraceutical ingredient for preventing obesity.

並列關鍵字

high-fat diet ； rat ； quercetin ； lycopene ； taurine ； litchi flower extract

參考文獻

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