羅漢果水萃物不易形成體脂肪功效之探討

Investigating the effect of Siraitia grosvenorii water extract on body fat accumulation

何政勳(Cheng-Hsun Ho)

指導教授 ： 吳亮宜

中原大學/理學院/生物科技研究所/碩士(2017年)

https://doi.org/10.6840/cycu201700829

摘要

因應社會的變遷與飲食上的改變，漸漸的肥胖之發生率也逐年上漲。然而當肥胖發生時，容易對身體造成許多不良的影響，因此肥胖已成為全球所關注的重要議題。羅漢果為傳統藥用植物，具預防高血糖、抗氧化和降低血脂等功效，且可活化在調控能量及平衡醣類和脂質代謝上之重要角色-AMPK，因此本實驗欲測試羅漢果水萃物在高油脂飲食誘導肥胖大鼠模式中對體脂肪形成之影響。
實驗將雄性Wistar大鼠分成四組，分別為餵食一般商業飼料之基礎組(B)，餵食40%高油脂飼料之控制組 (C)，以及餵食含0.4%羅漢果水萃物高油脂飼料之低劑量羅漢果水萃物組 (L)和餵食含0.8%羅漢果水萃物高油脂飼料之高劑量羅漢果水萃物組 (H)。實驗維持8週，實驗期間每週測量體重並分析禁食血漿三酸甘油酯及膽固醇濃度。動物犧牲後，收集血漿進行生化分析、秤量脂肪組織、收集肝臟與糞便檢測其脂質含量。
結果顯示，C組之體重、血漿及肝臟三酸甘油酯與膽固醇含量和腹部脂肪重量皆顯著高於B組，表示餵食高油脂飼料成功誘發肥胖、肝臟脂質累積與血脂異常之情形。經羅漢果水萃物處理後，在L組之部分，動物體重、血漿三酸甘油酯含量與C組無顯著差異；而肝臟三酸甘油酯與膽固醇含量則均顯著低於C組。而H組之部分，大鼠體重、血漿三酸甘油酯和膽固醇濃度及皮下脂肪重量與C組無顯著差異；而副睪脂和腸繫膜脂肪重、肝臟三酸甘油酯與膽固醇含量則顯著低於C組。此外，H組之糞便三酸甘油酯與膽固醇含量亦顯著高於C組，推測羅漢果水萃物可以干擾腸道對脂質的吸收或增加脂質的排出，以減少體脂肪及肝臟脂質含量的增加。由上述結果可知羅漢果水萃物具有改善體脂肪與肝臟脂質堆積之功效。

關鍵字

肥胖 ； 羅漢果 ； 高油脂飲食 ； 體重 ； 腹部脂肪

並列摘要

Due to change in social patterns, the incidence of obesity increased year by year. Obesity is a serious disease that can negatively impact on many systems of body. That is why obesity has recognized as a serious public issue. Siraitia grosvenorii is a traditional medicinal plant and it has been reported to prevent hyperglycemia, anti-oxidation and lower blood lipids, also can activate AMPK which regulate the energy and balance the carbohydrate and lipid metabolism. In this study, the effects of Siraitia grosvenorii water extract on high-fat diet (HFD)-induced obese rat of body fat accumulation were investigated.
The male Wistar rats were divided into four group: basal group (B), fed with chow diet; control group (C), fed with high fat diet alone; low dose group (L), fed with high fat diet plus 0.4% Siraitia grosvenorii water extract (SGWE); high dose group (L), fed with high fat diet plus 0.8% SGWE. The study last for 8 weeks. The fasting plasma triglyceride (TG) and cholesterol (TC) were measured during the experiment. After sacrifice, plasma was collected for biochemical analysis. Adipose tissue and liver were dissected and weighted. Lipid content of liver and fecal were also detected.
The data showed that group C had significant increases in body weight, visceral fat mass, plasma TG, TC, liver TG and TC as compared with group B. These results indicated that high fat feeding could induce obesity, dyslipidemia and liver lipid accumulation. There was no significant difference for body weight, plasma TG between group L and C. Similar results were also found between group L and B. But liver TG and TC in group L were significantly lower than group C. In group H, body weight, plasma TG, TC and subcutaneous fat weight had no difference with that of group C. Similar results were also found between group H and B. The weight of epididymal fat, mesenteric fat depots, the content of liver TG and TC in group H was significantly lower than those in group C. Moreover, fecal TG and TC in group H were higher than those in group C significantly. We suggested that SGWE might could interfere the lipid absorption and/or increase the excretion of lipid in the intestinal track, which cause the decreases of body fat and liver lipid contents. Therefore, SGWE could ameliorate body fat and liver lipid accumulation.

並列關鍵字

Obesity ； Siraitia grosvenorii ； high fat diet ； body weight ； visceral fat mass

參考文獻

林弘育、鍾成沛、江文章。(2012)。荷葉乙醇萃取物及其正丁醇區分層對以高熱量飲食誘導SD大鼠不易形成體脂肪之影響及其DNA微陣列分析。台灣農業化學與食品科學。50(5/6)，239-248。

林育靖、黃麗卿。(2005)。肥胖機轉的最新研究發展。基層醫學。20(8)，187-193。

陳冠宇。(2005)。心理治療有效減輕心因性肥胖患者之體重。北市醫學雜誌。2(3)，305-310.

關羽峻。(2009)。大豆蛋白對於餵食高膽固醇飲食倉鼠之血脂質與肝臟脂質的影響。台北醫學大學保健營養學研究所碩士論文。

周佩萱。(2014)。槲皮素複合補充劑對高脂飲食誘導肥胖大鼠之不易形成體脂肪作用及其分子機制探討。中山醫學大學營養學系碩士論文。