大豆蛋白對於餵食高膽固醇飲食倉鼠之血脂質與肝臟脂質的影響

Effects of soy protein on plasma and hepatic lipid in hamsters fed with high cholesterol diet

關羽峻(Yu-Chung Kwan 關羽峻)

指導教授 ： 陳俊榮

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2009年)

https://doi.org/10.6831/TMU.2009.00152

摘要

摘 要
本研究的目的是探討在高膽固醇飲食條件下，市售大豆蛋白(MIKI Protein 95)對於倉鼠之血脂質與肝臟脂質的影響。將24隻雄性倉鼠隨機分為三組：H 組 (19.7% 酪蛋白)、S1 組 (5% 大豆蛋白 +　14.7% 酪蛋白)以及 S2 組 (10% 大豆蛋白 + 9.7% 酪蛋白)。實驗為期八週，所有飼料中均添加 0.5% 膽固醇。結果發現：飲食中以大豆蛋白取代50% 酪蛋白作為蛋白質來源時，可顯著降低血漿總膽固醇、LDL-C 濃度及肝臟中三酸甘油酯濃度，同時可減少肝臟中膽固醇堆積的情況；然而在血液中三酸甘油酯濃度方面，則不具有改善效果。此外，在糞便的中性固醇與膽酸排出量方面，各組間並無顯著差異。由結果推論，市售大豆蛋白雖可能因加工過程導致蛋白質變性或添加其他配方物質，而影響部分生理活性，但飲食中攝取大豆蛋白，仍可有效降低血脂質與肝臟脂質濃度，因此認為可用於心血管疾病之預防與保健。

關鍵詞：大豆蛋白、高膽固醇血症、脂質代謝

關鍵字

大豆蛋白 ； 高膽固醇血症 ； 脂質代謝

並列摘要

Abstract
The aim of this study is to investigate the effects of commercial soy protein on plasma and hepatic lipid in hamster fed high cholesterol diet. Twenty-four hamsters were divided into three groups: H group (19.7% casein), S1 group (5% commercial soy protein + 14.7% casein), and S2 group (10% commercial soy protein + 9.7% casein). All groups were fed 0.5% cholesterol diet. After the 8-week experimental period, the results showed that soy protein could significantly lower plasma total cholesterol and LDL-C concentrations. Liver cholesterol and triglyceride contents were also significantly lower than H group (p < 0.05). However, there were no differences between fecal excretion of neutral steroids and bile acids of each group. In conclusion, commercial soy protein may lower plasma and hepatic lipid that may be useful for prevention of cardiovascular disease.

Key words: Soy protein, hypercholesterolemic, lipid metabolism

並列關鍵字

Soy protein ； hypercholesterolemic ； lipid metabolism

參考文獻

Anderson JW, Johnstone BM and Cook Newell ME (1995) Meta-analysis of the effects of soy protein intake on serum lipids. New Engl J Med 333:276-282.

Anderson JJ, Anthony MS, Cline JM, Washburn SA and Garner SC (1999) Health potential of soy isoflavones for menopausal women. Publ Health Nutr 2:489-504.

Ascencio C, Torres N, Isoard-Acosta, Gόmez-Pérez FJ, Hernández-Pando R and Tovar AR (2004) Soy protein affects serum insulin and hepatic SREBP-1 mRNA and reduces fatty liver in rats. J Nutr 134:522-529.

Bakhit RM, Klein BP and Essex-Sorlie D (1994) Intake of 25 g of soybean protein with or without soybean fiber alters plasma lipids in men with elevated cholesterol concentrations. J Nutr 124:213-222.

Banz Wj, Davis J, Peterson R and Iqbal MJ (2004) Gene expression and adipocity are modified by soy protein in male Zucker diabetic fatty rat. Obes. Res. 12:1907-1913.